Olympus FV1000 Measurement, Light Stimulation, Multi-Dimensional Time-Lapse, 3D Mosaic Imaging

Intensity

2,400 CH1

2,200

2,000

1,800 CH2

1,600

1,400

1,200 CH1

1,000

CH2

800

600

400

200

15,000	20,000	25,000	30,000	35,000	40,000
			Time (ms)

Application

Measurement

Diffusion measurement and molecular interaction analysis.

Light Stimulation

FRAP/FLIP/Photoactivation/Photoconversion/Uncaging.

Multi-Dimensional Time-Lapse

Long-term and multiple point.

3D Mosaic Imaging

High resolution images stitched to cover a large area.

Multi-Color Imaging

Full range of laser wavelengths for imaging of diverse fluorescent dyes and proteins.

3D/4D Volume Rendering

One-click 3D/4D image construction from acquired

XYZ/T images.

Change the angle of 3D image with a single click.

Colocalization

Configurable threshold values for fluorescence intensities on the scatterplot.

Accurate colocalization statistics and visualization of colocalized area on image.

FRET

Configuration wizard simplifies the setting of FRET experimental procedures.

Optimal laser excitation wavelengths for CFP/YFP FRET.

Image of variations in calcium concentration of HeLa cells expressing YC3.60 when stimulated with histamine.

Reference:

Takeharu Nagai, Shuichi Yamada, Takashi Tominaga, Michinori Ichikawa, and Atsushi Miyawaki 10554-10559, PNAS, July 20, 2004, vol. 101, no.29

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