Contents
Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual
General Instructions
Safety concept
Important information
Symbols used
Provide clarity
Action
Description
Safety regulations
Designated use
Non-intended use
Transport EC Declaration of Conformity
Disposal
Legal regulations
Light sources safety instructions
Lamp housing
During maintenance work on the supply unit
Supply unit
Mercury lamp
Table of Contents
Leica LED5000 MCI Leica LED5000 RL
Microscope Carrier AX Objective Nosepiece
Different Types of Objectives
Leica PSC Controller
Care, Maintenance, Contact Persons 113
109
IsoPro Motorized XY Stage 110
Leica M Series
Congratulations
Limits are made to be broken
Step Towards Infinity
Fewer cables
Reliability for your experiments
Have a special request? Let us know
Modular Design Everything is Relative
Objectives
Maximum Compatibility
Tubes
Eyepieces
On We Go
Assembly
Assembling the column adapter Assembling the focusing drive
Base and Focusing Column
Tools used
Hex socket screwdriver, 3 mm
Hex socket screwdriver, 4 mm
Optics carrier
No tools required
Tube
Preparations
Assembling the tube
Magnification range
Eyepieces
Tools used Preparation
Objective
bly and disassembly so that it does not Instructions on
Larly to the 2× planapochromatic objective
Remove the protective cap on the optics
Assembly
Objective Nosepiece Assembly
Do not fall onto the stage plate
Remove the transport anchor from the objective nosepiece
Tighten the locking screws
Objective Nosepiece Adjusting Parfocality
Adjustment
AX Carrier Preparation
Check the switch position
Tools
Hex socket screwdriver, 2.5 mm
Hex socket screwdriver, 4 mm Philips screwdriver
AX Carrier Assembly
Transmitted-light Base TL ST
Unpacking the base Focusing drive and column
Anchors as follows
Removing the transport anchors
Transmitted-light Base TL Bfdf
Stage assembly
Standard delivery
Standard stage
TL RC / TL RCI
Left or right operation
IsoPro Manual Cross-stage Assembly
IsoPro Cross-stage
Place the cross-stage on the base
Control assembly
Cross-stage assembly
Focusing drive and column
Basics
IsoPro Motorized Cross-stage Assembly
IsoPro Motorized Cross-stage Assembly
Damaged Place the cross-stage on the base
Bases
Cross-stage and base
Cross-stage for X-Y Stage Control Module
Terminals
Cables Terminals
Interface to the optics carrier is on the column
Feeding the cables
Cables Cable Duct
Alternative installation
Cables Diagram
Hold the LED5000 MCI with one
Leica LED5000 MCI
Hand and tightly screw the retraining Be facing downwards
Stirrups on both top holes on the drive hous Ing
Installation on the column
Leica LED5000 MCI Alternative Assembly
Must be facing upwards
Leica LED5000 RL
Into place
Column
Quick Start Guide
Fastest Route to Success
Overview of an M series Microscope
Correct Interpupillary Distance
Adjusting the Interpupillary Distance
Reference value
Look into the eyepieces
Dioptric correction
Using the Eyepieces
If you do not wear glasses
If you wear glasses
Focusing drive can be operated either left- or right-handed
Focusing
Focusing
Coarse/fine adjustment
Adjusting the resistance
Adjusting the resistance of the focus drive
Look into the eyepieces Focus on the specimen
Changing the magnification zoom
Zooming
Magnifications and fields of view
Ratchet Steps and Magnification Levels
Enabling and disabling ratchet steps
Requirements for parfocal work
Parfocality More Comfort and Convenience for Your Work
Parfocality
Enlarge the view to the maximum level Focus on the specimen
Opening the Iris Diaphragm
Iris Diaphragm
Closing the iris diaphragm
Eyepieces
Following eyepieces are available for the M series
Magnification Factors of the Eyepieces
Leica partner
Health Notes
Potential sources of infection
Dioptric correction
Using the Dioptric Correction
Dioptric Correction and Parfocality
Adjusting
Use
Graticules
Use with the AX carrier
Convergence angle in the stereoscopic image
Photography & Video
Adapter
Photography & Video
Leica DFC cameras
Leica Application Suite
Photo tubes and C-mounts
Trinocular video/phototube 50%
Trinocular video/phototube 100%
Switchover
Microscope Carrier
Stereoscopic viewing
Microscope Carrier AX
Compatibility
Coaxial light and the Leica M205 C
Parallax-free images
Parfocal work
Objective Nosepiece
Objective change
Technical constraints
Objectives Optical accessories
Achromatic objectives
Different Types of Objectives
Planachromatic objective 1×
Achromatic objectives with a long focal length
Bases
Transmitted-light Base TL ST Controls
Light intensity control
Transmitted-light Base TL ST Operation
Transmitted-light control
Bright field
Changing the Halogen Lamp
Transmitted-light Base TL ST Lamp Replacement
Precautionary measures
Transmitted-light Base TL Bfdf Controls
Adapter at the focusing drive
Light source
Transmitted-light Base TL Bfdf Operation
Dark field
Turn the control as far as it will go towards DF dark field
TL RC / TL RCI Controls
Inverted world?
TL RCI The Deflection Mirror
Tilted mirror
Functions of the rotary knob
Using a USB mouse only TL RCI
TL RCI Color Intensity and Temperature
TL RC / TL RCI Operation
Setting the relief contrast
Vertical bright-field illumination
TL RCI Methods in Transmitted Light
Single-sided dark field
Suitable for fixed specimens and fine struc- tures
Starting position
TL RCI Relief Images
Positive relief contrast
Negative relief contrast
Suitable for semitransparent and transparent specimens
Dynamic relief contrast
Daylight filter for TL ST
Using Filters
Filters for TL RC and TL RCI
Operating the cross-stage IsoPro
IsoPro Non-motorized Controls
Stage DCI module
IsoPro Motorized Controls
IsoPro
System Illumination
Beneath the MCI
Use Contact with the base
Bottom end of the guide rods
Antees the reproducibility of an experiment
Use the key to switch the illumination on or off
Using the keyboard
Illumination scenarios
Maximum brightness
Constraint
Accessories
Connection
Leica PSC Controller
Passing over the specimen quickly
Fine control of the cross-stage
Dimensional Drawings
Leica M125 / M165 C
292 217 431 537 154 106 356 430 340
292 217 547 116 385 154154 340 390 440
Leica M series Manual 100
Leica M205 C
Leica M series Manual 102
Leica M series Manual 103
Leica M series Manual 104
Leica M series Manual 105
Specifications for the Bases
Connections Power plug, power switch Weight
Ambient temperature 10-40 C
Energy consumption 30 W max
Illumination types
Concave mirror *** With external light source
Internal shutter/lamp control Yes Integrated filter holder
Concave mirror **** With external light source
Active f=10 mm, end tube f=13 mm Weight
Leica TL RC Leica TL RCI
Compatibility
IsoPro Motorized XY Stage
Appendix
Calculation example magnification in binocular tube
Parameter
Example
Warranty benefits
Care, Maintenance, Contact Persons
Contact address
Mail contact
Cleaning polymer components
Keep accessories in a dust-free place when not in use
Protection from dirt
Dust and dirt will affect the quality of your results