Leica M205 C, M205 A, M165 C manual Closing the iris diaphragm, Opening the Iris Diaphragm

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Iris Diaphragm

The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter: it regulates the avail- able light, which changes the depth of field. The "depth of field" is the area of a specimen that is brought into sharp focus.

Closing the iris diaphragm

★★ Close the iris diaphragm by turning the knob to the left. The subject appears darker and the depth of field increases.

Opening the Iris Diaphragm

★★ Open the iris diaphragm by turning the knob to the right. The subject now appears brighter, but the depth of field decreases.

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Contents Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Symbols used Important informationProvide clarity ActionSafety regulations DescriptionDesignated use Non-intended useTransport EC Declaration of Conformity DisposalLegal regulations Light sources safety instructions During maintenance work on the supply unit Lamp housingSupply unit Mercury lampTable of Contents Leica LED5000 MCI Leica LED5000 RL Microscope Carrier AX Objective NosepieceDifferent Types of Objectives Care, Maintenance, Contact Persons 113 Leica PSC Controller109 IsoPro Motorized XY Stage 110Leica M Series Congratulations Step Towards Infinity Limits are made to be brokenReliability for your experiments Fewer cablesModular Design Everything is Relative Have a special request? Let us knowMaximum Compatibility ObjectivesTubes EyepiecesOn We Go Assembly Base and Focusing Column Assembling the column adapter Assembling the focusing driveTools used Hex socket screwdriver, 3 mmOptics carrier Hex socket screwdriver, 4 mmTube No tools requiredPreparations Assembling the tubeMagnification range EyepiecesTools used Preparation  bly and disassembly so that it does not Instructions on ObjectiveLarly to the 2× planapochromatic objective Remove the protective cap on the opticsObjective Nosepiece Assembly AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepieceTighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Check the switch position AX Carrier PreparationTools Hex socket screwdriver, 2.5 mmAX Carrier Assembly Hex socket screwdriver, 4 mm Philips screwdriverUnpacking the base Focusing drive and column Transmitted-light Base TL STRemoving the transport anchors Anchors as followsStage assembly Transmitted-light Base TL BfdfStandard delivery Standard stageTL RC / TL RCI Left or right operation IsoPro Manual Cross-stage AssemblyIsoPro Cross-stage Place the cross-stage on the base Control assemblyCross-stage assembly Focusing drive and column IsoPro Motorized Cross-stage Assembly BasicsIsoPro Motorized Cross-stage Assembly Damaged Place the cross-stage on the base BasesCross-stage and base Cross-stage for X-Y Stage Control Module Terminals Cables TerminalsInterface to the optics carrier is on the column Cables Cable Duct Feeding the cablesCables Diagram Alternative installationLeica LED5000 MCI Hold the LED5000 MCI with oneHand and tightly screw the retraining Be facing downwards Stirrups on both top holes on the drive hous IngLeica LED5000 MCI Alternative Assembly Installation on the columnLeica LED5000 RL Must be facing upwardsInto place ColumnQuick Start Guide Fastest Route to Success Overview of an M series Microscope Adjusting the Interpupillary Distance Correct Interpupillary DistanceReference value Look into the eyepiecesUsing the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glassesFocusing Focusing drive can be operated either left- or right-handedFocusing Coarse/fine adjustmentAdjusting the resistance of the focus drive Adjusting the resistanceLook into the eyepieces Focus on the specimen Changing the magnification zoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimenOpening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M seriesLeica partner Health NotesPotential sources of infection Using the Dioptric Correction Dioptric correctionAdjusting Dioptric Correction and ParfocalityGraticules UseUse with the AX carrier Convergence angle in the stereoscopic imagePhotography & Video Photography & Video AdapterLeica DFC cameras Leica Application SuitePhoto tubes and C-mounts Trinocular video/phototube 50% Switchover Trinocular video/phototube 100%Microscope Carrier Stereoscopic viewing Microscope Carrier AXCompatibility Parallax-free images Coaxial light and the Leica M205 CObjective Nosepiece Parfocal workObjective change Technical constraintsObjectives Optical accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthBases Transmitted-light Base TL ST Controls Transmitted-light Base TL ST Operation Light intensity controlTransmitted-light control Bright fieldChanging the Halogen Lamp Transmitted-light Base TL ST Lamp ReplacementPrecautionary measures Adapter at the focusing drive Transmitted-light Base TL Bfdf ControlsTransmitted-light Base TL Bfdf Operation Light sourceDark field Turn the control as far as it will go towards DF dark fieldTL RC / TL RCI Controls TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knobTL RCI Color Intensity and Temperature Using a USB mouse only TL RCISetting the relief contrast TL RC / TL RCI OperationTL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- turesTL RCI Relief Images Starting positionPositive relief contrast Negative relief contrastDynamic relief contrast Suitable for semitransparent and transparent specimensDaylight filter for TL ST Using FiltersFilters for TL RC and TL RCI IsoPro Non-motorized Controls Operating the cross-stage IsoProStage DCI module IsoPro Motorized ControlsIsoPro System Illumination Use Contact with the base Beneath the MCIBottom end of the guide rods Antees the reproducibility of an experimentUsing the keyboard Use the key to switch the illumination on or offIllumination scenarios Maximum brightnessConstraint Accessories Leica PSC Controller ConnectionPassing over the specimen quickly Fine control of the cross-stageDimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Ambient temperature 10-40 C Connections Power plug, power switch WeightEnergy consumption 30 W max Illumination typesInternal shutter/lamp control Yes Integrated filter holder Concave mirror *** With external light sourceConcave mirror **** With external light source Active f=10 mm, end tube f=13 mm WeightLeica TL RC Leica TL RCI IsoPro Motorized XY Stage CompatibilityAppendix Calculation example magnification in binocular tube ParameterExample Care, Maintenance, Contact Persons Warranty benefitsContact address Mail contactKeep accessories in a dust-free place when not in use Cleaning polymer componentsProtection from dirt Dust and dirt will affect the quality of your results