Leica M205 C, M205 A, M165 C manual TL RC / TL RCI Operation, Setting the relief contrast

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TL RC™ / TL RCI™: Operation

Setting the relief contrast

The two switches on the left side of the trans- mitted-light base TL RC™/TL RCI™ activate two built-in flaps. The outer switch (1) controls the inverted relief contrast, and the inner switch (2) adjusts the positive relief contrast.

Depending on the flap position, a part of the opening of the built-in Fresnel lenses are covered, which results in the different contrast effects. Phase structures typically act as spatial, relief-type images—in the positive relief contrast like hills, in the inverted relief contrast like valleys.

Increased contrast without relief is attained if both diaphragms are set to 45°. A gap-like illuminated area is created. By tilting the deflec- tion mirror slightly, you can move the gap over the entire field of view and quickly toggle between positive and negative relief images. The dynamic effect makes it easy to distinguish phase structures from amplitude structures.

1

2

3

1Switch for adjusting the inverted relief contrast

2Switch for adjusting the positive relief contrast

3Deflection mirror

Leica M series

Manual

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Contents Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Action Symbols usedImportant information Provide clarityNon-intended use Safety regulationsDescription Designated useTransport EC Declaration of Conformity DisposalLegal regulations Light sources safety instructions Mercury lamp During maintenance work on the supply unitLamp housing Supply unitTable of Contents Leica LED5000 MCI Leica LED5000 RL Microscope Carrier AX Objective NosepieceDifferent Types of Objectives IsoPro Motorized XY Stage 110 Care, Maintenance, Contact Persons 113Leica PSC Controller 109Leica M Series Congratulations Limits are made to be broken Step Towards InfinityFewer cables Reliability for your experimentsHave a special request? Let us know Modular Design Everything is RelativeEyepieces Maximum CompatibilityObjectives TubesOn We Go Assembly Hex socket screwdriver, 3 mm Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Tools usedHex socket screwdriver, 4 mm Optics carrierAssembling the tube TubeNo tools required PreparationsMagnification range EyepiecesTools used Preparation Remove the protective cap on the optics  bly and disassembly so that it does not Instructions onObjective Larly to the 2× planapochromatic objectiveRemove the transport anchor from the objective nosepiece Objective Nosepiece AssemblyAssembly Do not fall onto the stage plateTighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Hex socket screwdriver, 2.5 mm Check the switch positionAX Carrier Preparation ToolsHex socket screwdriver, 4 mm Philips screwdriver AX Carrier AssemblyTransmitted-light Base TL ST Unpacking the base Focusing drive and columnAnchors as follows Removing the transport anchorsStandard stage Stage assemblyTransmitted-light Base TL Bfdf Standard deliveryTL RC / TL RCI Left or right operation IsoPro Manual Cross-stage AssemblyIsoPro Cross-stage Place the cross-stage on the base Control assemblyCross-stage assembly Focusing drive and column Basics IsoPro Motorized Cross-stage AssemblyIsoPro Motorized Cross-stage Assembly Damaged Place the cross-stage on the base BasesCross-stage and base Cross-stage for X-Y Stage Control Module Terminals Cables TerminalsInterface to the optics carrier is on the column Feeding the cables Cables Cable DuctAlternative installation Cables DiagramStirrups on both top holes on the drive hous Ing Leica LED5000 MCIHold the LED5000 MCI with one Hand and tightly screw the retraining Be facing downwardsInstallation on the column Leica LED5000 MCI Alternative AssemblyColumn Leica LED5000 RLMust be facing upwards Into placeQuick Start Guide Fastest Route to Success Overview of an M series Microscope Look into the eyepieces Adjusting the Interpupillary DistanceCorrect Interpupillary Distance Reference valueIf you wear glasses Using the EyepiecesDioptric correction If you do not wear glassesCoarse/fine adjustment FocusingFocusing drive can be operated either left- or right-handed FocusingAdjusting the resistance Adjusting the resistance of the focus driveLook into the eyepieces Focus on the specimen Changing the magnification zoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Enlarge the view to the maximum level Focus on the specimen Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work ParfocalityOpening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the EyepiecesLeica partner Health NotesPotential sources of infection Dioptric correction Using the Dioptric CorrectionDioptric Correction and Parfocality AdjustingConvergence angle in the stereoscopic image GraticulesUse Use with the AX carrierPhotography & Video Leica Application Suite Photography & VideoAdapter Leica DFC camerasPhoto tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% SwitchoverMicroscope Carrier Stereoscopic viewing Microscope Carrier AXCompatibility Coaxial light and the Leica M205 C Parallax-free imagesTechnical constraints Objective NosepieceParfocal work Objective changeObjectives Optical accessories Achromatic objectives with a long focal length Different Types of ObjectivesAchromatic objectives Planachromatic objective 1×Bases Transmitted-light Base TL ST Controls Bright field Transmitted-light Base TL ST OperationLight intensity control Transmitted-light controlChanging the Halogen Lamp Transmitted-light Base TL ST Lamp ReplacementPrecautionary measures Transmitted-light Base TL Bfdf Controls Adapter at the focusing driveTurn the control as far as it will go towards DF dark field Transmitted-light Base TL Bfdf OperationLight source Dark fieldTL RC / TL RCI Controls Functions of the rotary knob TL RCI The Deflection MirrorInverted world? Tilted mirrorUsing a USB mouse only TL RCI TL RCI Color Intensity and TemperatureTL RC / TL RCI Operation Setting the relief contrastSuitable for fixed specimens and fine struc- tures TL RCI Methods in Transmitted LightVertical bright-field illumination Single-sided dark fieldNegative relief contrast TL RCI Relief ImagesStarting position Positive relief contrastSuitable for semitransparent and transparent specimens Dynamic relief contrastDaylight filter for TL ST Using FiltersFilters for TL RC and TL RCI Operating the cross-stage IsoPro IsoPro Non-motorized ControlsStage DCI module IsoPro Motorized ControlsIsoPro System Illumination Antees the reproducibility of an experiment Use Contact with the baseBeneath the MCI Bottom end of the guide rodsMaximum brightness Using the keyboardUse the key to switch the illumination on or off Illumination scenariosConstraint Accessories Fine control of the cross-stage Leica PSC ControllerConnection Passing over the specimen quicklyDimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Illumination types Ambient temperature 10-40 CConnections Power plug, power switch Weight Energy consumption 30 W maxConcave mirror *** With external light source Internal shutter/lamp control Yes Integrated filter holderConcave mirror **** With external light source Active f=10 mm, end tube f=13 mm WeightLeica TL RC Leica TL RCI Compatibility IsoPro Motorized XY StageAppendix Calculation example magnification in binocular tube ParameterExample Mail contact Care, Maintenance, Contact PersonsWarranty benefits Contact addressDust and dirt will affect the quality of your results Keep accessories in a dust-free place when not in useCleaning polymer components Protection from dirt