Leica M165 C, M205 A manual Parameter, Example, Calculation example magnification in binocular tube

Page 112

Calculating the Total Magnification and Field of View Diameter

Parameter

MO	Magnification of objective

ME	Magnification of eyepiece

z	Magnification changer position

qTube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™

rFactor 1.25x if the planachromatic and planapochromatic objectives of the MZ125/MZ16 are used on the MS5, MZ6, MZ75 or MZ95

NFOV Field number of the eyepiece. Field numbers are printed on the eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.

Example

MO	1× objective

ME	25×/9.5 eyepiece

z	Zoom position 4

q	Coaxial reflected light 1.5×, tube factor

r	Factor 1.25×

Calculation example: magnification in binocular tube		Calculation example: field of view diameter in the specimen

MTOT VIS = MO × ME × z × q × r		NFOV
or		NFOV
or		∅ OF:
1 × 25 × 4 × 1.5 × 1.25 = 187.5×		MO × z ×q ×r
1 × 25 × 4 × 1.5 × 1.25 = 187.5×

Leica M series	Manual	112

Image 112

Contents Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Symbols used Important informationProvide clarity ActionSafety regulations DescriptionDesignated use Non-intended useLegal regulations DisposalTransport EC Declaration of Conformity Light sources safety instructions During maintenance work on the supply unit Lamp housingSupply unit Mercury lampTable of Contents Different Types of Objectives Microscope Carrier AX Objective NosepieceLeica LED5000 MCI Leica LED5000 RL Care, Maintenance, Contact Persons 113 Leica PSC Controller109 IsoPro Motorized XY Stage 110Leica M Series Congratulations Step Towards Infinity Limits are made to be brokenReliability for your experiments Fewer cablesModular Design Everything is Relative Have a special request? Let us knowMaximum Compatibility ObjectivesTubes EyepiecesOn We Go Assembly Base and Focusing Column Assembling the column adapter Assembling the focusing driveTools used Hex socket screwdriver, 3 mmOptics carrier Hex socket screwdriver, 4 mmTube No tools requiredPreparations Assembling the tubeTools used Preparation EyepiecesMagnification range  bly and disassembly so that it does not Instructions on ObjectiveLarly to the 2× planapochromatic objective Remove the protective cap on the opticsObjective Nosepiece Assembly AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepieceAdjustment Objective Nosepiece Adjusting ParfocalityTighten the locking screws Check the switch position AX Carrier PreparationTools Hex socket screwdriver, 2.5 mmAX Carrier Assembly Hex socket screwdriver, 4 mm Philips screwdriverUnpacking the base Focusing drive and column Transmitted-light Base TL STRemoving the transport anchors Anchors as followsStage assembly Transmitted-light Base TL BfdfStandard delivery Standard stageTL RC / TL RCI IsoPro Cross-stage IsoPro Manual Cross-stage AssemblyLeft or right operation Cross-stage assembly Control assemblyPlace the cross-stage on the base Focusing drive and column IsoPro Motorized Cross-stage Assembly BasicsIsoPro Motorized Cross-stage Assembly Cross-stage and base BasesDamaged Place the cross-stage on the base Cross-stage for X-Y Stage Control Module Interface to the optics carrier is on the column Cables TerminalsTerminals Cables Cable Duct Feeding the cablesCables Diagram Alternative installationLeica LED5000 MCI Hold the LED5000 MCI with oneHand and tightly screw the retraining Be facing downwards Stirrups on both top holes on the drive hous IngLeica LED5000 MCI Alternative Assembly Installation on the columnLeica LED5000 RL Must be facing upwardsInto place ColumnQuick Start Guide Fastest Route to Success Overview of an M series Microscope Adjusting the Interpupillary Distance Correct Interpupillary DistanceReference value Look into the eyepiecesUsing the Eyepieces Dioptric correctionIf you do not wear glasses If you wear glassesFocusing Focusing drive can be operated either left- or right-handedFocusing Coarse/fine adjustmentAdjusting the resistance of the focus drive Adjusting the resistanceZooming Changing the magnification zoomLook into the eyepieces Focus on the specimen Enabling and disabling ratchet steps Ratchet Steps and Magnification LevelsMagnifications and fields of view Parfocality More Comfort and Convenience for Your Work Requirements for parfocal workParfocality Enlarge the view to the maximum level Focus on the specimenClosing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M seriesPotential sources of infection Health NotesLeica partner Using the Dioptric Correction Dioptric correctionAdjusting Dioptric Correction and ParfocalityGraticules UseUse with the AX carrier Convergence angle in the stereoscopic imagePhotography & Video Photography & Video AdapterLeica DFC cameras Leica Application SuitePhoto tubes and C-mounts Trinocular video/phototube 50% Switchover Trinocular video/phototube 100%Microscope Carrier Compatibility Microscope Carrier AXStereoscopic viewing Parallax-free images Coaxial light and the Leica M205 CObjective Nosepiece Parfocal workObjective change Technical constraintsObjectives Optical accessories Different Types of Objectives Achromatic objectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthBases Transmitted-light Base TL ST Controls Transmitted-light Base TL ST Operation Light intensity controlTransmitted-light control Bright fieldPrecautionary measures Transmitted-light Base TL ST Lamp ReplacementChanging the Halogen Lamp Adapter at the focusing drive Transmitted-light Base TL Bfdf ControlsTransmitted-light Base TL Bfdf Operation Light sourceDark field Turn the control as far as it will go towards DF dark fieldTL RC / TL RCI Controls TL RCI The Deflection Mirror Inverted world?Tilted mirror Functions of the rotary knobTL RCI Color Intensity and Temperature Using a USB mouse only TL RCISetting the relief contrast TL RC / TL RCI OperationTL RCI Methods in Transmitted Light Vertical bright-field illuminationSingle-sided dark field Suitable for fixed specimens and fine struc- turesTL RCI Relief Images Starting positionPositive relief contrast Negative relief contrastDynamic relief contrast Suitable for semitransparent and transparent specimensFilters for TL RC and TL RCI Using FiltersDaylight filter for TL ST IsoPro Non-motorized Controls Operating the cross-stage IsoProIsoPro IsoPro Motorized ControlsStage DCI module System Illumination Use Contact with the base Beneath the MCIBottom end of the guide rods Antees the reproducibility of an experimentUsing the keyboard Use the key to switch the illumination on or offIllumination scenarios Maximum brightnessConstraint Accessories Leica PSC Controller ConnectionPassing over the specimen quickly Fine control of the cross-stageDimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Ambient temperature 10-40 C Connections Power plug, power switch WeightEnergy consumption 30 W max Illumination typesInternal shutter/lamp control Yes Integrated filter holder Concave mirror *** With external light sourceLeica TL RC Leica TL RCI Active f=10 mm, end tube f=13 mm WeightConcave mirror **** With external light source IsoPro Motorized XY Stage CompatibilityAppendix Example ParameterCalculation example magnification in binocular tube Care, Maintenance, Contact Persons Warranty benefitsContact address Mail contactKeep accessories in a dust-free place when not in use Cleaning polymer componentsProtection from dirt Dust and dirt will affect the quality of your results