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Contents Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Provide clarity Symbols usedImportant information ActionDesignated use Safety regulationsDescription Non-intended useTransport EC Declaration of Conformity DisposalLegal regulations Light sources safety instructions Supply unit During maintenance work on the supply unitLamp housing Mercury lampTable of Contents Leica LED5000 MCI Leica LED5000 RL Microscope Carrier AX Objective NosepieceDifferent Types of Objectives 109 Care, Maintenance, Contact Persons 113Leica PSC Controller IsoPro Motorized XY Stage 110Leica M Series Congratulations Step Towards Infinity Limits are made to be brokenReliability for your experiments Fewer cablesModular Design Everything is Relative Have a special request? Let us knowTubes Maximum CompatibilityObjectives EyepiecesOn We Go Assembly Tools used Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Hex socket screwdriver, 3 mmOptics carrier Hex socket screwdriver, 4 mmPreparations TubeNo tools required Assembling the tubeMagnification range EyepiecesTools used Preparation Larly to the 2× planapochromatic objective  bly and disassembly so that it does not Instructions onObjective Remove the protective cap on the opticsDo not fall onto the stage plate Objective Nosepiece AssemblyAssembly Remove the transport anchor from the objective nosepieceTighten the locking screws Objective Nosepiece Adjusting ParfocalityAdjustment Tools Check the switch positionAX Carrier Preparation Hex socket screwdriver, 2.5 mmAX Carrier Assembly Hex socket screwdriver, 4 mm Philips screwdriverUnpacking the base Focusing drive and column Transmitted-light Base TL STRemoving the transport anchors Anchors as followsStandard delivery Stage assemblyTransmitted-light Base TL Bfdf Standard stageTL RC / TL RCI Left or right operation IsoPro Manual Cross-stage AssemblyIsoPro Cross-stage Place the cross-stage on the base Control assemblyCross-stage assembly Focusing drive and column IsoPro Motorized Cross-stage Assembly BasicsIsoPro Motorized Cross-stage Assembly Damaged Place the cross-stage on the base BasesCross-stage and base Cross-stage for X-Y Stage Control Module Terminals Cables TerminalsInterface to the optics carrier is on the column Cables Cable Duct Feeding the cablesCables Diagram Alternative installationHand and tightly screw the retraining Be facing downwards Leica LED5000 MCIHold the LED5000 MCI with one Stirrups on both top holes on the drive hous IngLeica LED5000 MCI Alternative Assembly Installation on the columnInto place Leica LED5000 RLMust be facing upwards ColumnQuick Start Guide Fastest Route to Success Overview of an M series Microscope Reference value Adjusting the Interpupillary DistanceCorrect Interpupillary Distance Look into the eyepiecesIf you do not wear glasses Using the EyepiecesDioptric correction If you wear glassesFocusing FocusingFocusing drive can be operated either left- or right-handed Coarse/fine adjustmentAdjusting the resistance of the focus drive Adjusting the resistanceLook into the eyepieces Focus on the specimen Changing the magnification zoomZooming Magnifications and fields of view Ratchet Steps and Magnification LevelsEnabling and disabling ratchet steps Parfocality Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Enlarge the view to the maximum level Focus on the specimenOpening the Iris Diaphragm Iris DiaphragmClosing the iris diaphragm Eyepieces Magnification Factors of the Eyepieces Following eyepieces are available for the M seriesLeica partner Health NotesPotential sources of infection Using the Dioptric Correction Dioptric correctionAdjusting Dioptric Correction and ParfocalityUse with the AX carrier GraticulesUse Convergence angle in the stereoscopic imagePhotography & Video Leica DFC cameras Photography & VideoAdapter Leica Application SuitePhoto tubes and C-mounts Trinocular video/phototube 50% Switchover Trinocular video/phototube 100%Microscope Carrier Stereoscopic viewing Microscope Carrier AXCompatibility Parallax-free images Coaxial light and the Leica M205 CObjective change Objective NosepieceParfocal work Technical constraintsObjectives Optical accessories Planachromatic objective 1× Different Types of ObjectivesAchromatic objectives Achromatic objectives with a long focal lengthBases Transmitted-light Base TL ST Controls Transmitted-light control Transmitted-light Base TL ST OperationLight intensity control Bright fieldChanging the Halogen Lamp Transmitted-light Base TL ST Lamp ReplacementPrecautionary measures Adapter at the focusing drive Transmitted-light Base TL Bfdf ControlsDark field Transmitted-light Base TL Bfdf OperationLight source Turn the control as far as it will go towards DF dark fieldTL RC / TL RCI Controls Tilted mirror TL RCI The Deflection MirrorInverted world? Functions of the rotary knobTL RCI Color Intensity and Temperature Using a USB mouse only TL RCISetting the relief contrast TL RC / TL RCI OperationSingle-sided dark field TL RCI Methods in Transmitted LightVertical bright-field illumination Suitable for fixed specimens and fine struc- turesPositive relief contrast TL RCI Relief ImagesStarting position Negative relief contrastDynamic relief contrast Suitable for semitransparent and transparent specimensDaylight filter for TL ST Using FiltersFilters for TL RC and TL RCI IsoPro Non-motorized Controls Operating the cross-stage IsoProStage DCI module IsoPro Motorized ControlsIsoPro System Illumination Bottom end of the guide rods Use Contact with the baseBeneath the MCI Antees the reproducibility of an experimentIllumination scenarios Using the keyboardUse the key to switch the illumination on or off Maximum brightnessConstraint Accessories Passing over the specimen quickly Leica PSC ControllerConnection Fine control of the cross-stageDimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Energy consumption 30 W max Ambient temperature 10-40 CConnections Power plug, power switch Weight Illumination typesInternal shutter/lamp control Yes Integrated filter holder Concave mirror *** With external light sourceConcave mirror **** With external light source Active f=10 mm, end tube f=13 mm WeightLeica TL RC Leica TL RCI IsoPro Motorized XY Stage CompatibilityAppendix Calculation example magnification in binocular tube ParameterExample Contact address Care, Maintenance, Contact PersonsWarranty benefits Mail contactProtection from dirt Keep accessories in a dust-free place when not in useCleaning polymer components Dust and dirt will affect the quality of your results