Leica M205 A, M165 C, M205 C manual TL RCI The Deflection Mirror, Inverted world?, Tilted mirror

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TL RCI™: The Deflection Mirror

1

Inverted world?

Depending on the properties of the specimen (refractive index of the environment) and the perception of the viewer, it is possible that the switches described below for positive and inverted relief contrast are to be operated in reverse. This means that the lower switch, rather than of the upper switch, controls inverted relief contrast and vice-versa.

Tilted mirror

The built-in mirror features one flat and one concave side and can be rotated and moved. The concave side has been specially designed for the optical requirements of objectives with a high numerical aperture. The black rotary knob on the left side of the transmitted-light base can be used to rotate the built-in path-folding mirror and move it forwards/back.

The concave cutout on the handle indicates the concave side of the mirror, allowing intuitive operation at any time without eye contact.

The angle of light incidence in the specimen plane changes depending on the tilt and posi- tion of the mirror. As a result, switching between transmitted light bright field, oblique illumina- tion and dark field-like illumination is possible.

Functions of the rotary knob

The rotary knob (1) fulfills the following tasks:

Turning the deflection mirror from the flat to the concave side.

Slight tilting to guide the light beam through the specimen plane at a steeper or flatter angle

Moving the deflection mirror (forwards/ back)

Leica M series

Manual

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Contents Leica M125 Leica M165 C Leica M205 C Leica M205 a Manual General Instructions Safety concept Important information Symbols usedProvide clarity ActionDescription Safety regulationsDesignated use Non-intended useDisposal Legal regulationsTransport EC Declaration of Conformity Light sources safety instructions Lamp housing During maintenance work on the supply unitSupply unit Mercury lampTable of Contents Microscope Carrier AX Objective Nosepiece Different Types of ObjectivesLeica LED5000 MCI Leica LED5000 RL Leica PSC Controller Care, Maintenance, Contact Persons 113109 IsoPro Motorized XY Stage 110Leica M Series Congratulations Limits are made to be broken Step Towards InfinityFewer cables Reliability for your experimentsHave a special request? Let us know Modular Design Everything is RelativeObjectives Maximum CompatibilityTubes EyepiecesOn We Go Assembly Assembling the column adapter Assembling the focusing drive Base and Focusing ColumnTools used Hex socket screwdriver, 3 mmHex socket screwdriver, 4 mm Optics carrierNo tools required TubePreparations Assembling the tubeEyepieces Tools used PreparationMagnification range Objective  bly and disassembly so that it does not Instructions onLarly to the 2× planapochromatic objective Remove the protective cap on the opticsAssembly Objective Nosepiece AssemblyDo not fall onto the stage plate Remove the transport anchor from the objective nosepieceObjective Nosepiece Adjusting Parfocality AdjustmentTighten the locking screws AX Carrier Preparation Check the switch positionTools Hex socket screwdriver, 2.5 mmHex socket screwdriver, 4 mm Philips screwdriver AX Carrier AssemblyTransmitted-light Base TL ST Unpacking the base Focusing drive and columnAnchors as follows Removing the transport anchorsTransmitted-light Base TL Bfdf Stage assemblyStandard delivery Standard stageTL RC / TL RCI IsoPro Manual Cross-stage Assembly IsoPro Cross-stageLeft or right operation Control assembly Cross-stage assemblyPlace the cross-stage on the base Focusing drive and column Basics IsoPro Motorized Cross-stage AssemblyIsoPro Motorized Cross-stage Assembly Bases Cross-stage and baseDamaged Place the cross-stage on the base Cross-stage for X-Y Stage Control Module Cables Terminals Interface to the optics carrier is on the columnTerminals Feeding the cables Cables Cable DuctAlternative installation Cables DiagramHold the LED5000 MCI with one Leica LED5000 MCIHand and tightly screw the retraining Be facing downwards Stirrups on both top holes on the drive hous IngInstallation on the column Leica LED5000 MCI Alternative AssemblyMust be facing upwards Leica LED5000 RLInto place ColumnQuick Start Guide Fastest Route to Success Overview of an M series Microscope Correct Interpupillary Distance Adjusting the Interpupillary DistanceReference value Look into the eyepiecesDioptric correction Using the EyepiecesIf you do not wear glasses If you wear glassesFocusing drive can be operated either left- or right-handed FocusingFocusing Coarse/fine adjustmentAdjusting the resistance Adjusting the resistance of the focus driveChanging the magnification zoom ZoomingLook into the eyepieces Focus on the specimen Ratchet Steps and Magnification Levels Enabling and disabling ratchet stepsMagnifications and fields of view Requirements for parfocal work Parfocality More Comfort and Convenience for Your WorkParfocality Enlarge the view to the maximum level Focus on the specimenIris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factors of the EyepiecesHealth Notes Potential sources of infectionLeica partner Dioptric correction Using the Dioptric CorrectionDioptric Correction and Parfocality AdjustingUse GraticulesUse with the AX carrier Convergence angle in the stereoscopic imagePhotography & Video Adapter Photography & VideoLeica DFC cameras Leica Application SuitePhoto tubes and C-mounts Trinocular video/phototube 50% Trinocular video/phototube 100% SwitchoverMicroscope Carrier Microscope Carrier AX CompatibilityStereoscopic viewing Coaxial light and the Leica M205 C Parallax-free imagesParfocal work Objective NosepieceObjective change Technical constraintsObjectives Optical accessories Achromatic objectives Different Types of ObjectivesPlanachromatic objective 1× Achromatic objectives with a long focal lengthBases Transmitted-light Base TL ST Controls Light intensity control Transmitted-light Base TL ST OperationTransmitted-light control Bright fieldTransmitted-light Base TL ST Lamp Replacement Precautionary measuresChanging the Halogen Lamp Transmitted-light Base TL Bfdf Controls Adapter at the focusing driveLight source Transmitted-light Base TL Bfdf OperationDark field Turn the control as far as it will go towards DF dark fieldTL RC / TL RCI Controls Inverted world? TL RCI The Deflection MirrorTilted mirror Functions of the rotary knobUsing a USB mouse only TL RCI TL RCI Color Intensity and TemperatureTL RC / TL RCI Operation Setting the relief contrastVertical bright-field illumination TL RCI Methods in Transmitted LightSingle-sided dark field Suitable for fixed specimens and fine struc- turesStarting position TL RCI Relief ImagesPositive relief contrast Negative relief contrastSuitable for semitransparent and transparent specimens Dynamic relief contrastUsing Filters Filters for TL RC and TL RCIDaylight filter for TL ST Operating the cross-stage IsoPro IsoPro Non-motorized ControlsIsoPro Motorized Controls IsoProStage DCI module System Illumination Beneath the MCI Use Contact with the baseBottom end of the guide rods Antees the reproducibility of an experimentUse the key to switch the illumination on or off Using the keyboardIllumination scenarios Maximum brightnessConstraint Accessories Connection Leica PSC ControllerPassing over the specimen quickly Fine control of the cross-stageDimensional Drawings Leica M125 / M165 C 292 217 431 537 154 106 356 430 340 292 217 547 116 385 154154 340 390 440 Leica M series Manual 100 Leica M205 C Leica M series Manual 102 Leica M series Manual 103 Leica M series Manual 104 Leica M series Manual 105 Specifications for the Bases Connections Power plug, power switch Weight Ambient temperature 10-40 CEnergy consumption 30 W max Illumination typesConcave mirror *** With external light source Internal shutter/lamp control Yes Integrated filter holderActive f=10 mm, end tube f=13 mm Weight Leica TL RC Leica TL RCIConcave mirror **** With external light source Compatibility IsoPro Motorized XY StageAppendix Parameter ExampleCalculation example magnification in binocular tube Warranty benefits Care, Maintenance, Contact PersonsContact address Mail contactCleaning polymer components Keep accessories in a dust-free place when not in useProtection from dirt Dust and dirt will affect the quality of your results