GE Q HP, SP HP manual

HiTrap SP HP, 1 ml and 5 ml

HiTrap Q HP, 1 ml and 5 ml

HiTrap™ SP HP and HiTrap Q HP are prepacked, read y to use cation and ani on

exchange columns for met hod scouting, gr oup separation s, sample concentr ation

and sample clean-up of ch arged biomolec ules. HiTrap SP HP and HiTrap Q HP p rovide

fast, reproducible, a nd easy separatio ns in a convenient for mat.

The columns can be operat ed with a syringe, p eristaltic pum p or liquid

chromatography system such as ÄKTAdesign™ or FPLC™ System.

Contents

Main Instructions 71-7149-00 AN HiTrap ion exchange columns GE Healthcare HiTrap SP HP, HiTrap Q HP, Connectorkit Table of contents 1. Description Media properties Column p. 4 Table 1. HiTrap SP HP and HiTrap Q HP column s characteris tics 2. Selection of ion exchanger and conditions Selection of bu er pH and ionic strength Strategy 1. Binding and elution of all sample components Strategy 2. Enrichment of target protein Strategy 3. Binding of sample contaminants Start bu er Starting pH p. 8 Table 3. Buffers for anion exchange chromatography. Fig 2. Recommended buffer s ubstances for ca tion exchange c hromatograph y. pH 3. Operation Bu er preparation Sample preparation p. 10 Table 4. Prepacked column s for desalting an d buffer exchang e. Puri cation 4. Optimization Further optimization 5. Choice of gradient type Elution with stepwise ionic strength gradients Elution with continuous ionic strength gradients 6. Determination of binding capacity Page 7. Scaling up 8. Storage 9. Ordering Information Page Page Elanders stervla 2006