p. 10 Table 4. Prepacked column s for desalting an d buffer exchang e.Code No Column Loading volume Elution volume C omments Application
17-1408-01 Hi Trap 0.1–1.5 ml 1.3–4.0 ml Prepacked with For desaltin g and buffer
Desalting Sephadex™ G-25 exchange of protein
Superfine. Requires extrac ts (Mr>5000).
a syringe or pump
to run.
17-5087-01 HiP rep Up to 15–20 ml Prepacked with For desalting and
26/10 15 ml Sephadex G -25 buffer exchange of
Desalting Fine. Requires a protein extracts
pump to run. (Mr>5000).
17-0851-01 PD -10 2.5 ml 3.5 ml Pr epacked with For desalting and
Desalting Sephadex G-25. buffer exchange
Requires only of protein extracts
gravity to run. (Mr>5000).
17-0855-01 NICK™ 0.1 ml 0.4 ml Pre packed with For separation of
Sephadex G-25. proteins (Mr>5000)
and nicktranslated
DNA from radiolabelled
nucleotides not shorter
than 120 me rs, and
similar separations.
17-0853-01 NAP ™-5 0.5 ml 1.0 ml Prepacked wi th For purification of
17-0854-01 NAP-10 1.0 m l 1.5 ml Sephadex G-25 proteins (M r>5000),
17-0852-01 NAP -25 2.5 ml 3.5 ml DNA grade. DNA and oligo-
Requires only nu cleotides greater
gravity t o run. than 10 bases in le ngth.