p. 2
Code No. Designation No. supplied
17-1151-01 HiTrap SP HP 5 x 1 ml
17-1152-01 HiTrap SP HP 5 x 5 ml
17-1153-01 HiTrap Q HP 5 x 1 ml
17-1154-01 HiTrap Q HP 5 x 5 ml

Connectorkit

Connectors supplied Usage No. supplied
1/16” male/luer female Connection of syrin ge to top of
HiTrap column 1
Tubing connector Connection of tu bing (e.g. Peri staltic
flangeless/M6 fem ale Pump P1) to bottom of HiTrap co lumn* 1
Tubing connector Connection of tu bing (e.g. Peri staltic
flangeless/M6 ma le Pump P1) to top of HiTrap column** 1
Union 1/16” female/ Connection to orig inal FPLC System
M6 male through bottom of HiTrap co lumn 1
Union M6 female/ Connec tion to origin al FPLC System
1/16” male through top of HiTrap column 1
Stop plug female, 1/16” Sealing bottom of HiTrap column 2, 5 or 7
* Union 1/16” female/M6 male is a lso needed.
** Union M6 femal e/1/16” male is also n eeded.
Table of contents
1. Description 3
2. Selection of ion excha nger and conditi ons 5
3. Operation 9
4. Optimization 12
5. Choice of gradient type 13
6. Determination of binding capacity 15
7. Scaling up 17
8. Storage 17
9. Ordering Information 17