p. 8
Table 3. Buffers for anion exchange chromatography.
pH interval Substance Conc. (mM) Counter-ion pKa (25 °C)1
4.3–5.3 N-Methylpiperazine 20 Cl- 4.75
4.8–5.8 Piperazine 20 Cl- or HCOO- 5.33
5.5–6.5 L-Histidine 20 Cl- 6.04
6.0–7.0 bis-Tris 20 Cl- 6.48
6.2–7.2; 8.6–9.6 b is-Tris propane 20 Cl- 6.65; 9.10
7.3–8.3 Triethanolamine 20 Cl - or CH3COO- 7.76
7.6–8 .6 Tris 20 Cl- 8.07
8.0–9.0 N-Methyldiethanolamine 20 SO4
2- 8.52
8.0–9.0 N-Methyldiethanolamine 50 Cl- or CH3COO- 8.52
8.4–9.4 Diethanolam ine 20 at pH 8 .4 Cl- 8.88
50 at pH 8.8
8.4–9.4 Propane 1,3-Diamino 20 Cl- 8.88
9.0–10.0 Ethanolamine 20 Cl- 9.50
9.2–10.2 Piperazine 20 Cl- 9.73
10.0–11.0 Propane 1,3-Diamino 20 Cl- 10.55
10.6–11.6 Piperi dine 20 Cl - 11.12
1 Ref: Handbook of chem istry and phy sics, 83rd edition, CRC, 2002–2003.
pH