Assembling Carefully turn and tighten the 2⋅ planapochromatic objective clockwise to 2⋅ planapochromatic the limit stop without applying excessive force.
Assembling Raise the focusing drive with the drive heads to objective nosepiece provide sufficient space for the 2⋅ planapochro-
matic objective.
Fit the objective nosepiece like a microscope car- rier with the Allen screw (see page 34, position 1).
Insert the optics carrier in the objective nosepiece.
Retighten the clamping screw.
Attach the limit stop to the column of the stand so that the working distance beneath the 2⋅ objective will always measure approx. 15 mm.
Adjusting parfocal • Fit and adjust the 1⋅ and 2⋅ planapochromatic objectives as described. The image then remains parfocal in the range of < 0.05 mm, and refocusing after changing the objective is no longer required.
•Use a very fine, flat object (e.g. an object micrometer) for the adjustment, which allows maximum focusing even at highest resolution.
Screw the 2⋅ planapochromatic objective into the beam path.
Adjusting the diopter settings and parfocality (see p. 20).
Screw the 1⋅ planapochromatic objective into the beam path.
Observe the same object with smallest magnifica- tion without changing the settings made with the 2⋅ planapochromatic objective.
Turn the 1⋅ planapochromatic objective counter- clockwise until the object can be seen in focus at the smallest magnification.
Slowly zoom to the highest magnification. At the same time correct the focus by turning the 1⋅ planapochromatic objective.
•Verify that the object is in focus at highest magnification with the
2⋅ planapochromatic as well as the 1⋅ planapochromatic objective without refocusing. Otherwise turn the objective at the ring nut until
the object is focused.
Fasten the ring nut with both screws.
Do not overtighten both screws.
Leica M Stereomicroscopes – Assembly | 37 |