Leica M165 C B, M125 B user manual Dioptric Correction and Parfocality, Adjusting

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Dioptric Correction and Parfocality

Leica stereomicroscopes are parfocally matched. The prerequisite for this is the correct setting of the diopters and the parfocality. The following adjustments have to be carried out only once by each user.

Preparations

Move the lever of the video/phototube to the "observation" position and open the diaphragm.

Adjusting

1.Set the dioptric correction for both eyepieces to "0".

2.Select the lowest magnification and focus on a flat specimen.

3.Select the highest magnification and read- just the sharpness.

4.Select the lowest magnification again, but do not look into the eyepieces.

6.Rotate the eyepieces counterclockwise in the "+" direction as far as they will go (+5 diopter settings).

7.Look into the eyepieces.

8.Slowly rotate each eyepiece individually in the "–" direction until each eye sees the object sharply imaged.

9.Select the highest magnification and refo- cus if necessary.

Now, if you adjust the magnification from the lowest to the highest level, the specimen is always brought into sharp focus. If not, repeat the process.

Leica M series

User Manual

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Contents Living up to Life Copyright Leica M125 B nameplate Risks arising from, using the microscope forServicing Use in clean roomsCleaning General NotesImportant Safety Notes Explanatory notes Symbols UsedImportant information DisposalNon-intended use Safety InstructionsDescription Intended use of instrumentIntegration in third-party products Repairs, service workOnly original Leica Microsystems spare parts may be used TransportCareful handling Legal regulationsEC Declaration of Conformity Health risksColumn External power supply for the focusingLeica M165 C B Contents Different Types of Objectives Care, Maintenance, Contact PersonsLeica TL5000 Ergo Transmitted Light Base Leica M Series Congratulations Step Towards Infinity Fewer cables Tubes Maximum CompatibilityObjectives All new objectives of the Leica M series are parfocalOn We Go Assembly Allen key, 3 mm Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Tools usedGroove Optics carrierTools used Assembling the Optics Carrier Allen key, 4 mm Place the optics carrier on the focusPreparations TubeTools used No tools requiredTools used Preparation EyepiecesMagnification range Preparation ObjectiveAttaching the objective Base is delivered with the adapter plate Unpacking the base Focusing drive and columnBottom using the 4 Allen screws TL5000 Ergo transmitted light baseLater Wiring Connections Leica M165 C BNew Leica M series features extensive Terminal for the 50-watt power supply providedFeeding the cables Cables Cable DuctQuick Start Guide Fastest Route to Success Overview of an M Series Microscope Look into the eyepieces Adjusting the interpupillary distanceCorrect interpupillary distance Reference valuesIf you wear glasses Using the EyepiecesDioptric Correction If you do not wear glassesCoarse/fine adjustment FocusingFocusing drive can be operated either left- or right-handed FocusingAdjusting the resistance Adjusting the Resistance of the Focus DriveZooming Changing the Magnification ZoomLook into the eyepieces Focus on the specimen Enabling and disabling click stops Click Stops and MagnificationsMagnifications and fields of view Enlarge the view to the maximum level Focus on the specimen Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work ParfocalityClosing the iris diaphragm Iris DiaphragmOpening the Iris Diaphragm Eyepieces Following eyepieces are available for the M series Magnification Factor of the EyepiecesPotential sources of infection Health NotesDioptric Correction Using the Dioptric CorrectionDioptric Correction and Parfocality AdjustingUse GraticulesObjectives Optical Accessories Achromatic objectives with a long focal length Different Types of ObjectivesAchromatic objectives Planachromatic objective 1×Bases Control for the size of the aperture/ balance Leica TL5000 Ergo Transmitted Light Base ControlsLeica TL5000 Ergo Transmitted Light Base Operation Switching the transmitted light base onOff Leica TL5000 Ergo Transmitted Light Base Operation Technical Data Connections Power supplyLeica TL5000 Ergo Transmitted Light Base Light sourceAppendix Example ParameterCalculation example magnification in the binocular tube Warranty benefits Care, Maintenance, Contact PersonsCare Dust and dirt will affect the quality of your results Keep accessories in a dust-free place when not in useCleaning polymer components Protection from dirt