Leica M125 B Parameter, Example, Calculation example magnification in the binocular tube

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Calculating the Total Magnification and Field of View Diameter

Parameter

MO	Magnification of objective

ME	Magnification of eyepiece

z	Magnification changer position

qTube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° ErgoTube™

rFactor 1.25× if the planachromatic and planapochromatic objectives of the MZ125/MZ16 are used on the MS5, MZ6, MZ75 or MZ95

NFOV Field number of the eyepiece. Field numbers are printed on the eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.

Example

MO	1× objective

ME	25×/9.5 eyepiece

z	Zoom position 4

q	Coaxial incident light 1.5×, tube factor

r	Factor 1.25×

Calculation example: magnification in the binocular tube		Calculation example: field of view diameter in the specimen

MTOT VIS = MO × ME × z × q × r		NFOV
or		NFOV
or		∅ OF:
1 × 25 × 4 × 1.5 × 1.25 = 187.5×		MO × z ×q ×r
1 × 25 × 4 × 1.5 × 1.25 = 187.5×

Leica M series	User Manual	54

Image 54

Contents Living up to Life Copyright Risks arising from, using the microscope for Leica M125 B nameplateGeneral Notes Use in clean roomsCleaning ServicingImportant Safety Notes Disposal Symbols UsedImportant information Explanatory notesIntended use of instrument Safety InstructionsDescription Non-intended useTransport Repairs, service workOnly original Leica Microsystems spare parts may be used Integration in third-party productsHealth risks Legal regulationsEC Declaration of Conformity Careful handlingExternal power supply for the focusing ColumnLeica M165 C B Contents Care, Maintenance, Contact Persons Different Types of ObjectivesLeica TL5000 Ergo Transmitted Light Base Leica M Series Congratulations Step Towards Infinity Fewer cables All new objectives of the Leica M series are parfocal Maximum CompatibilityObjectives TubesOn We Go Assembly Tools used Base and Focusing ColumnAssembling the column adapter Assembling the focusing drive Allen key, 3 mmAllen key, 4 mm Place the optics carrier on the focus Optics carrierTools used Assembling the Optics Carrier GrooveNo tools required TubeTools used PreparationsEyepieces Tools used PreparationMagnification range Objective PreparationAttaching the objective TL5000 Ergo transmitted light base Unpacking the base Focusing drive and columnBottom using the 4 Allen screws Base is delivered with the adapter plateTerminal for the 50-watt power supply provided Wiring Connections Leica M165 C BNew Leica M series features extensive LaterCables Cable Duct Feeding the cablesQuick Start Guide Fastest Route to Success Overview of an M Series Microscope Reference values Adjusting the interpupillary distanceCorrect interpupillary distance Look into the eyepiecesIf you do not wear glasses Using the EyepiecesDioptric Correction If you wear glassesFocusing FocusingFocusing drive can be operated either left- or right-handed Coarse/fine adjustmentAdjusting the Resistance of the Focus Drive Adjusting the resistanceChanging the Magnification Zoom ZoomingLook into the eyepieces Focus on the specimen Click Stops and Magnifications Enabling and disabling click stopsMagnifications and fields of view Parfocality Parfocality More Comfort and Convenience for Your WorkRequirements for parfocal work Enlarge the view to the maximum level Focus on the specimenIris Diaphragm Closing the iris diaphragmOpening the Iris Diaphragm Eyepieces Magnification Factor of the Eyepieces Following eyepieces are available for the M seriesHealth Notes Potential sources of infectionUsing the Dioptric Correction Dioptric CorrectionAdjusting Dioptric Correction and ParfocalityGraticules UseObjectives Optical Accessories Planachromatic objective 1× Different Types of ObjectivesAchromatic objectives Achromatic objectives with a long focal lengthBases Leica TL5000 Ergo Transmitted Light Base Controls Control for the size of the aperture/ balanceSwitching the transmitted light base on Leica TL5000 Ergo Transmitted Light Base OperationOff Leica TL5000 Ergo Transmitted Light Base Operation Technical Data Light source Power supplyLeica TL5000 Ergo Transmitted Light Base ConnectionsAppendix Parameter ExampleCalculation example magnification in the binocular tube Care, Maintenance, Contact Persons Warranty benefitsCare Protection from dirt Keep accessories in a dust-free place when not in useCleaning polymer components Dust and dirt will affect the quality of your results

Parameter

Example

Calculation example: magnification in the binocular tube