Leica 106Z Adjusting the mercury vapour lamp, Figs.. a Bringing the discharge arc into focus

Page 15

Adjusting the mercury vapour lamp

This adjustment is important­ for obtaining a uniform­ light spot and good-quality fluorescence..

While adjusting the mirror image, do not project light on to the electrodes for long periods (risk of explosion­ through overheating)

The two electrodes are imaged as an extension of the plane of symmetry of the discharge arc, but are difficult to see..

Switch on the supply unit and wait for two or three minutes

• The 100W supply unit emits a whistle

Swing out the UV-light excluder (page 21)

Work without the UV-light excluder is only permitted for adju- stment, and then only for short periods

Pull out the light stop (see page 16, MZ10 F and page 22, Fluorescence module)..

MZ10 F: Turn the filter set in the beam path (page 16)..

Stereo-fluorescence module: Insert the excitation filter (page 22)..

Mark a cross on a piece­ of paper and place it in the middle of the illuminated spot

Select the lowest magnification­

The discharge arc is visible in the illuminated­ spot and lies

at about­ 45° to the lines of the cross (fig.. a)

Look into the eyepieces and focus on the cross

Using your unaided eye, observe the discharge­ arc on the paper and bring it into focus with the focusing knob (3) (fig.. a)

Using the positioning knobs (1 and 2), displace the discharge arc (fig.. b)

Bring the mirror image­ of the discharge arc into focus

with the knob (6) and use the knobs 4 and 5 to position­ it

symmetrically­ relative to the original­ image (fig.. c)

For the 50W burner, the discharge-arc image­ and its mirror image should touch

For the 100W burner, the discharge-arc image­ and its mirror image should be superimposed..

Using the focusing knob (3), readjust the illuminated field

The illuminated field should now be large, circular and as uniform as possible

Reposition the UV-light excluder correctly (page 21)..

Hg 50W

Hg 100W

Figs.. a: Bringing the discharge arc into focus

3

1

Figs.. b: Positioning the discharge­ arc relative to the cross

Figs.. c: Focusing and positioning the mirror image of the discharge arc

2

4

5 6

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Contents Leica Stereo Fluorescence Systems Page Leica stereo-fluorescence module Contents Leica MZ10 F Fluorescence stereomicroscopeDescription Safety conceptLight source Safety regulations Natural science Application Fluorescence filter set ApplicationsIndustry Application Fluorescence filter set Overview Leica MZ10 F Its components and controls3c 4a Filter sets for Leica MZ10 F Filter sets DesignationBarrier filter Assembly UV protection screen microscope carrier Leica MZ10 FAdditional components Video-/phototube Double-iris diaphragmAssembly Observe the safety regulations­ onFitting and changing the mercury-vapour burner Replacing the collector Lamp housing assembleStray-light protection Removing the collector and the heat-protection filterFuses Observe the safety regulations onFigs.. a Bringing the discharge arc into focus Adjusting the mercury vapour lampFluoiii filter system Use Dioptric correctionReflexes hot-spots 5a, 5b 4a 4b, 4c, 4d Outfit with stereo-fluorescence module ComponentsFilter sets for stereo-fluorescence module 13,14 Changing the filter set Stereo-fluorescence module Focusing drive base of standFluorescence module stereomicroscope Video-/phototubesAdditional assembly instructions UV-light excluder side-faced columnFilterholder fluorescence module Observation Stereo-fluorescence module Focusing and observingFocusing Transmitted light With the fluorescence illuminationLeica Microsystems the brand for outstanding products