Leica 106Z user manual Fluoiii filter system

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Leica MZ10 F

FLUOIII filter system

The filter system consists of a rapid filter changer for the barrier- and excitation filters and two slots for a light stop and for a filter slide respectively..

Rapid filter changer

The rapid filter changer of the Leica MZ10 F accepts a total of four sets of filters.. These are inscribed (see table on page 9).. Each filter set consists of two barrier filters for the observation beam paths and one excitation filter for the illumination beam path..

To avoid fingerprints on the filters in the filter sets, try to avoid touching them.. If fingerprints do occur, remove them with a clean lint-free cloth and pure alcohol..

An empty filter carrier is available for individual filter combinations..

Insert a set of filters into the rapid filter changer so that the inscription (e..g.. GFP) is upright and on the right-hand side.. Make sure that the shape of the filter set follows the shape of the instrument..

Turn the filter set until it engages..

Insert a total of four sets of filters, or insert dummy filter carriers if the number is less..

If you use less than four filter sets, you must insert in the free spaces the dummy filter carriers provided.

If you leave spaces unfilled, you risk eye damage from direct UV radiation from the third beam path

(see page 5).

Dummy filter carrier

In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters.. The third path has been closed.. Use the dummy filter carrier:

if you are using less than four sets of filters

if you are working without the fluorescence illuminator for periods of up to 15 seconds..

Do not use the dummy filter carrier to block the light from the mercury-vapour burner for more than 15 seconds, or it will heat up.. Use the light stop instead..

Light stop

The FLUOIII filter system includes a slot for the light stop.. In phases where the object is to be examined in e..g.. transmitted light instead of under conditions of fluorescence, the light stop is used to block the illumination beam path so that the mercury- vapour burner does not have its life shortened by being switched on and off more than is absolutely necessary (see page 5)..

Filter slide

Next to the light stop is a slot accepting a filter slide which can hold an individually-selectable filter, e..g.. a grey filter..

Working without the fluorescence illuminator

Use the light stop to block the light from the fluorescence illuminator..

Turn the dummy filter carrier in the observation beam

path so that there is no barrier filter in position which might cause false colour..

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Contents Leica Stereo Fluorescence Systems Page Contents Leica MZ10 F Fluorescence stereomicroscope Leica stereo-fluorescence moduleSafety concept DescriptionLight source Safety regulations Applications Natural science Application Fluorescence filter setIndustry Application Fluorescence filter set Leica MZ10 F Its components and controls Overview3c 4a Filter sets Designation Filter sets for Leica MZ10 FBarrier filter Assembly Leica MZ10 F Additional components Video-/phototubeDouble-iris diaphragm UV protection screen microscope carrierObserve the safety regulations­ on AssemblyFitting and changing the mercury-vapour burner Lamp housing assemble Stray-light protectionRemoving the collector and the heat-protection filter Replacing the collectorObserve the safety regulations on FusesAdjusting the mercury vapour lamp Figs.. a Bringing the discharge arc into focusFluoiii filter system Dioptric correction UseReflexes hot-spots Outfit with stereo-fluorescence module Components 5a, 5b 4a 4b, 4c, 4dFilter sets for stereo-fluorescence module 13,14 Stereo-fluorescence module Focusing drive base of stand Fluorescence module stereomicroscopeVideo-/phototubes Changing the filter setUV-light excluder side-faced column Additional assembly instructionsFilterholder fluorescence module Stereo-fluorescence module Focusing and observing Focusing Transmitted lightWith the fluorescence illumination ObservationLeica Microsystems the brand for outstanding products