Leica 106Z user manual Description, Safety concept

Page 4

Description

Fluorescence technique

Some substances fluoresce when irradiated with short-wave

light.. This property­ is utilized in the fluorescence technique, in which certain structures and features which do not fluo- rescence can be tagged with a fluorescing dye.. An example is ­green-fluorescing protein (GFP), which is used in molecular biology..

Stereo-fluorescence

With Leica fluorescence systems you can complete unprepared fluorescing specimens to be non-destructively studied in three

dimensions, manipulated,­ sorted and recorded­ .. The intense light emitted by the mercury-vapour burner, used in conjunction with selected filter sets, enables even the ­finest structures to be

differentiated­and expands the amount of information revealed by the incident-light fluorescence ­technique..

Fluorescence stereomicroscope

The Leica MZ10 F with 10:1 zoom is the routine stereo­ microscope for fluorescence applications.. The patented sepa- rate beam path (TriBeam™) for the fluorescence illuminator and the patented filter system (FLUOIII™) together produce excellent fluorescence images..

Stereo-fluorescence module

The stereo-fluorescence modul can be combined with the Leica stereomicroscope models MS5, MZ6, MZ7..5, MZ9..5, MZ12..5, MZ16, and with older models..

Safety concept

Before you set up the instrument and before you fit the fluorescence illuminator, read:

this user manual, observing the notes relating to safety

the user manual for your stereomicroscope, observing the notes relating to safety and care..

Permitted uses

Leica stereo-fluorescence systems are equipped with a special fluorescence illuminator.. They are intended for the three- dimensional observation of fluorescing objects.. They consist of:

a stereomicroscope with stand, binocular tube and eyepieces

an integrated FLUOIII filter system (for MZ10 F) or a separate stereo-fluorescence module

appropriate filter sets with barrier- and excitation filters, a light stop, and individually-selectable filters

a UV protection screen

a lamp housing with high-pressure mercury vapour burner

a supply unit with power cable..

Prohibited uses

The use of Leica stereo-fluorescence systems in a different manner from that described in this user manual can lead to inju- ry, malfunction and damage..

Do not fit different plugs or cables

Do not dismantle or modify components unless instructions for doing so are given in the user manual

Components may only be opened by authorized personnel..

User manual

The present user manual describes only the functions of the fluorescence illuminator, the use of the filter sets, and the fitting of the fluorescence module.. For detailed information about the use of the stereomicroscope, its care and its safety, please refer to those sections of the separate manual M2-105-0EN which relate to the Leica MZ12..5 with 12..5:1 zoom..

Place of use

Leica stereo-fluorescence systems are intended for use in closed rooms and may not be used outdoors..

Responsibilities of person in charge of instrument

Ensure that the Leica stereo-fluorescence systems are operated, maintained and repaired only by authorized and trained personnel

Ensure that personnel who use the instrument have read and understood this user manual and in particular all safety instructions..

4

Image 4
Contents Leica Stereo Fluorescence Systems Page Contents Leica MZ10 F Fluorescence stereomicroscope Leica stereo-fluorescence moduleSafety concept DescriptionLight source Safety regulations Applications Natural science Application Fluorescence filter setIndustry Application Fluorescence filter set Leica MZ10 F Its components and controls Overview3c 4a Filter sets Designation Filter sets for Leica MZ10 FBarrier filter Assembly Leica MZ10 F Additional components Video-/phototubeDouble-iris diaphragm UV protection screen microscope carrierObserve the safety regulations­ on AssemblyFitting and changing the mercury-vapour burner Lamp housing assemble Stray-light protectionRemoving the collector and the heat-protection filter Replacing the collectorObserve the safety regulations on FusesAdjusting the mercury vapour lamp Figs.. a Bringing the discharge arc into focusFluoiii filter system Dioptric correction UseReflexes hot-spots Outfit with stereo-fluorescence module Components 5a, 5b 4a 4b, 4c, 4dFilter sets for stereo-fluorescence module 13,14 Stereo-fluorescence module Focusing drive base of stand Fluorescence module stereomicroscopeVideo-/phototubes Changing the filter setUV-light excluder side-faced column Additional assembly instructionsFilterholder fluorescence module Stereo-fluorescence module Focusing and observing Focusing Transmitted lightWith the fluorescence illumination ObservationLeica Microsystems the brand for outstanding products