Leica 106Z Stereo-fluorescence module Focusing and observing, Focusing Transmitted light

Page 23

Use

Stereo-fluorescence module

Focusing and observing

Observe the safety regulations on page 4 and note the recommendations on page 5 for using the mercury- vapour burner. Position the UV-light excluder correctly (see page 22):

Hold the arm

Release the central

clamping screw

Position the UV-light excluder in front of the objective

Tighten the central clamping screw..

Focusing

Carry out the dioptric correction­ and the focusing in accordance with the user manual for the stereo­microscope, either:

in transmitted light:

pushing in the filter holder with diaphragm (3) to block the fluorescence­ illumination­ (see page 22).. Pull the filter holder with diaphragm out again before obser­ving the fluorescence..

3

with the fluorescence illumination:

pulling out the filter holder with diaphragm (3) (see page 22)

Insert the excitation filter (1) (page 22)

1 2 3

Switch on the supply unit (page 14).. For the 100W lamp, wait two or three minutes for the whistle signal..

Observation

First use the lowest magnification to observe the object

You will gain a better overall view and can more easily find

interesting­ features

Refocus slightly if necessary

Observe details at a higher magnification..

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Image 23
Contents Leica Stereo Fluorescence Systems Page Leica stereo-fluorescence module Contents Leica MZ10 F Fluorescence stereomicroscopeDescription Safety conceptLight source Safety regulations Natural science Application Fluorescence filter set ApplicationsIndustry Application Fluorescence filter set 3c 4a OverviewLeica MZ10 F Its components and controls Barrier filter Filter sets for Leica MZ10 FFilter sets Designation Assembly UV protection screen microscope carrier Leica MZ10 FAdditional components Video-/phototube Double-iris diaphragmFitting and changing the mercury-vapour burner AssemblyObserve the safety regulations­ on Replacing the collector Lamp housing assembleStray-light protection Removing the collector and the heat-protection filterFuses Observe the safety regulations onFigs.. a Bringing the discharge arc into focus Adjusting the mercury vapour lampFluoiii filter system Reflexes hot-spots UseDioptric correction 5a, 5b 4a 4b, 4c, 4d Outfit with stereo-fluorescence module ComponentsFilter sets for stereo-fluorescence module 13,14 Changing the filter set Stereo-fluorescence module Focusing drive base of standFluorescence module stereomicroscope Video-/phototubesFilterholder fluorescence module Additional assembly instructionsUV-light excluder side-faced column Observation Stereo-fluorescence module Focusing and observingFocusing Transmitted light With the fluorescence illuminationLeica Microsystems the brand for outstanding products