Dynex 91000051 manual Configuring the System, Sweep height? SHOW

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Sweep height? 370

Configuring the System

Remember if your protocol requires sweeping, that must first be specified in the plate definition used in the protocol.

11.Select YES to specify a sweep on all aspiration cycles. The display will read:

Sweep height? 370

SHOW

Note: The default value is displayed. Select SHOW to move the wash head to the default sweep position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed.

12.Enter the desired value using the keypad and press ENTER. The display will read:

Sweep stroke? 100

Note: There isn’t a SHOW function for the sweep stroke. Determine optimum sweep stroke setting by starting with a small number and increase it if the residual aspirate volume is not acceptable. If you run a protocol and the wash pins strike the sides or bottom of the well, reduce the sweep stroke and review your plate setup parameters.

13.Enter the desired value using the keypad and press ENTER. The display will read:

Bottom wash?

NO

YES

NO

14.Select YES if a bottom wash is to be included. The display will read:

Wash height? 368

SHOW

Note: The default value for bottom wash height is displayed. Select SHOW to move the wash head to the default bottom wash position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed.

Opsys MW™ User’s Guide

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Contents User’s Guide Please read carefully before using the Opsys MWOpsys MW Microplate Washer This manual is published by DYNEX Technologies, Inc Part No. 91000051, Revision BDYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA Tel. 703 800 288-2354 Fax. 703Warranty and Special Provisions Limited Warranty Page Table of Contents Table of ContentsChapter 2 Installation Chapter 3 ConfigurationChapter 8 Service Chapter 7 TroubleshootingIndex Opsys MW User’s GuideThis manual gives you the information needed to About this ManualAbout this Manual This page is intentionally left blank Description Chapter 1 IntroductionDescription Programming Description Wash HeadWash Head Wash Head Position Wash Head PositionsDescription Dispense HeightWaste Wash FluidOpsys MW User’s Guide Features FeaturesOpsys MW User’s Guide Dimensions and Weight SpecificationsPower Requirements SpecificationsStandards Input RangesPlate Setup Assay ProgrammingLabel Description Warning LabelsWarning Labels Opsys MW User’s Guide Unpacking Chapter 2 Installation UnpackingMaterials Provided To unpack the components Hardware Components Hardware ComponentsKeypad. The keypad is used by the operator to select commands and enter wash parameters Figure 3. Rear View of the Opsys MW System Connectors at the rear of the Opsys MW system are shown in FigurePositioning the Instrument Positioning the InstrumentInstalling the Wash Head Installing the Wash HeadInstalling the Wash Container Installing the Wash ContainerTo install the Wash Container Installing the Waste Container Installing the Waste ContainerTo install the Waste Container and Foam/Aerosol Trap Turning on the System Turning on the SystemSETUP PROGRAM 1. Connect the Opsys MW to the laboratory electrical supply outlet7 8 9 Chapter 3 Configuration The KeypadThe Keypad Purpose “Cancel” keyScroll keys If you are entering or editing information , aKeys AlphanumericThe numeral shown on the key is displayed by pressing the key once. If the key is pressed two toConfigure the Instrument SETUPMenus MenusThe PROGRAM Menu The SETUP MenuConfiguring the System Configuring the SystemCommand CLEANSpecifying the Wash Head Specifying Plate Parameters To specify parameters for a plate type Note The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed Sweep height? SHOW Opsys MW User’s Guide Displaying the Software Version Note Refer to page 74 for additional pump calibration instructions Specifying the Pump ParametersSelecting Clean Opsys MW User’s Guide Wash Protocol Operations Chapter 4 Wash Protocols Wash Protocol OperationsOperation PurgeFill DispenseSoak Creating a New Wash Protocol Creating a New Wash ProtocolSpecifying the Header To create a new wash protocol header Select plate 2 DYNEX1 7. Re-enter the password and press ENTER. The display will readVariable strips? YESNO 9. If YES is selected, the display will read Constant timing? YESNOSelect option MOVE PURGE Specifying Wash Cycle Operations To specify a Purge To specify a Move cycle Note An Aspirate or Dispense must be To specify an Aspiration within a MoveNote A Dispense or Aspirate must be performed within a Move cycle To specify a Dispense within a Move6. Press ENTER. The display will read Cycles? To specify a Soak within a Move Note A Fill must be performed within a Move cycle To specify a Fill within a MoveNote A volume ranging from 50 to 300 microliters can be entered Select option SOAK FILL Ending a MOVE Cycle2. Scroll right to display the next selection. The display will read Select option END LOOP 3. Select END LOOP. The display will readTo specify a Soak outside of a Move Select option SOAK END 2. Select END. The display will read 1 NAME01 To specify the End of the wash protocolModifying a Wash Protocol Modifying a Wash ProtocolTo modify a wash protocol MOVE To modify a wash operation To delete a wash operation To insert a wash operation Copying a Wash Protocol Copying a Wash ProtocolTo copy a wash protocol Overwrite test? NO YESNO 8. Select YES. The display will read 7 NAME04 SETUP PROGRAMPreparation Chapter 5 Washing a PlateTo empty the waste container PreparationTo refill the wash container Washing a Plate Washing a PlateTo wash a plate Opsys MW User’s Guide Daily maintenance Chapter 6 Routine Maintenance Routine Maintenance ProceduresWeekly maintenance Six month maintenanceAs required maintenance To clean the system Cleaning and DecontaminationCleaning and Decontamination To decontaminate the systemCleaning the Containers Cleaning the ContainersTo empty and clean the waste container Replacing the Tubing Replacing the TubingTo replace the dispense tubing To replace the vacuum tubing To replace the waste tubing Pump Calibration Pump CalibrationCalibration Protocol 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function refer to page Opsys MW User’s Guide Operational Problems Chapter 7 Troubleshooting Operational ProblemsSymptom ResolutionSymptom waste container is X Motor Home High Waste LevelZ Motor Home Test Too Large isTo remove the wash head assembly Chapter 8 Service Service ProceduresTo clean the wash head assembly To replace the wash head assemblySpare Parts AccessoriesMajor Assemblies HardwareReturning a Washer for Service Returning a Washer for ServiceTo return a Washer for service EQUIPMENT DECLARATION EQUIPMENT IN TRANSITCERTIFICATE OF DECONTAMINATION Suggested decontamination methodsDYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Contact DYNEX TechnologiesContact DYNEX Technologies Index Indexvariable strips, 41 warning labels, 11 Wash Container, 5