Dynex 91000051 manual Specifying Plate Parameters, Configuring the System

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Specifying Plate Parameters

Configuring the System

Specifying Plate Parameters

The PLATE command allows plate parameters to be specified for up to 10 plate types.

When setting plate parameters, a default value is displayed for each of the wash head positions. The default values are factory-set and represent approximate starting values for each wash head position.

Note: The wash head positions are described on page 4.

Increasing the value of a wash head position by one unit lowers the head

0.1mm and decreasing the numerical value of a wash head position by one unit raises the head 0.1 mm. (e.g. an increment of 20 steps equals one millimeter of vertical pin travel (+20 = 1mm up, -20 = 1mm down)).

The SHOW command is available when setting each wash head position. Select SHOW to move the wash head to the indicated position so that the height can be observed.

For example, when setting dispense height, the default value is 180:

DISP. HEIGHT? 180

SHOW

If, after selecting SHOW, the dispense pins are found to be too low by approximately 1.0 mm, the dispense height position can be raised by 1.0 mm by entering 170 for the dispense height.

When using the SHOW function when defining the aspirate height, if the aspirate pins touch the well bottom, increase the aspirate height. If you cannot move the plate slightly side-to-side and up-down without immediate interference from the aspirate pins, increase the aspirate height.

30	Opsys MW™ User’s Guide

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Contents User’s Guide Please read carefully before using the Opsys MWOpsys MW Microplate Washer DYNEX Technologies 14340 Sullyfield Circle Part No. 91000051, Revision BThis manual is published by DYNEX Technologies, Inc Chantilly, VA 20151-1683 USA Tel. 703 800 288-2354 Fax. 703Warranty and Special Provisions Limited Warranty Page Chapter 2 Installation Table of ContentsTable of Contents Chapter 3 ConfigurationIndex Chapter 7 TroubleshootingChapter 8 Service Opsys MW User’s GuideThis manual gives you the information needed to About this ManualAbout this Manual This page is intentionally left blank Description Chapter 1 IntroductionDescription Programming Description Wash HeadWash Head Description Wash Head PositionsWash Head Position Dispense HeightWash Fluid WasteOpsys MW User’s Guide Features FeaturesOpsys MW User’s Guide Power Requirements SpecificationsDimensions and Weight SpecificationsPlate Setup Input RangesStandards Assay ProgrammingLabel Description Warning LabelsWarning Labels Opsys MW User’s Guide Unpacking Chapter 2 Installation UnpackingMaterials Provided To unpack the components Hardware Components Hardware ComponentsKeypad. The keypad is used by the operator to select commands and enter wash parameters Connectors at the rear of the Opsys MW system are shown in Figure Figure 3. Rear View of the Opsys MW SystemPositioning the Instrument Positioning the InstrumentInstalling the Wash Head Installing the Wash HeadInstalling the Wash Container Installing the Wash ContainerTo install the Wash Container Installing the Waste Container Installing the Waste ContainerTo install the Waste Container and Foam/Aerosol Trap SETUP PROGRAM Turning on the SystemTurning on the System 1. Connect the Opsys MW to the laboratory electrical supply outlet7 8 9 Chapter 3 Configuration The KeypadThe Keypad Scroll keys “Cancel” keyPurpose If you are entering or editing information , aThe numeral shown on the key is displayed by AlphanumericKeys pressing the key once. If the key is pressed two toMenus SETUPConfigure the Instrument MenusThe SETUP Menu The PROGRAM MenuCommand Configuring the SystemConfiguring the System CLEANSpecifying the Wash Head Specifying Plate Parameters To specify parameters for a plate type Note The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed Sweep height? SHOW Opsys MW User’s Guide Displaying the Software Version Specifying the Pump Parameters Note Refer to page 74 for additional pump calibration instructionsSelecting Clean Opsys MW User’s Guide Operation Chapter 4 Wash Protocols Wash Protocol OperationsWash Protocol Operations PurgeFill DispenseSoak Creating a New Wash Protocol Creating a New Wash ProtocolSpecifying the Header To create a new wash protocol header Variable strips? YESNO 7. Re-enter the password and press ENTER. The display will readSelect plate 2 DYNEX1 9. If YES is selected, the display will read Constant timing? YESNOSelect option MOVE PURGE Specifying Wash Cycle Operations To specify a Purge To specify a Move cycle To specify an Aspiration within a Move Note An Aspirate or Dispense must beTo specify a Dispense within a Move Note A Dispense or Aspirate must be performed within a Move cycleSelect option ASPIRATE DISPENSE To specify a Soak within a Move Note A Fill must be performed within a Move cycle To specify a Fill within a MoveNote A volume ranging from 50 to 300 microliters can be entered 2. Scroll right to display the next selection. The display will read Ending a MOVE CycleSelect option SOAK FILL Select option END LOOP 3. Select END LOOP. The display will readTo specify a Soak outside of a Move To specify the End of the wash protocol Select option SOAK END 2. Select END. The display will read 1 NAME01Modifying a Wash Protocol Modifying a Wash ProtocolTo modify a wash protocol MOVE To modify a wash operation To delete a wash operation To insert a wash operation Copying a Wash Protocol Copying a Wash ProtocolTo copy a wash protocol 7 NAME04 SETUP PROGRAM Overwrite test? NO YESNO 8. Select YES. The display will readTo empty the waste container Chapter 5 Washing a PlatePreparation PreparationTo refill the wash container Washing a Plate Washing a PlateTo wash a plate Opsys MW User’s Guide Weekly maintenance Chapter 6 Routine Maintenance Routine Maintenance ProceduresDaily maintenance Six month maintenanceAs required maintenance Cleaning and Decontamination Cleaning and DecontaminationTo clean the system To decontaminate the systemCleaning the Containers Cleaning the ContainersTo empty and clean the waste container Replacing the Tubing Replacing the TubingTo replace the dispense tubing To replace the vacuum tubing To replace the waste tubing Pump Calibration Pump CalibrationCalibration Protocol 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function refer to page Opsys MW User’s Guide Symptom Chapter 7 Troubleshooting Operational ProblemsOperational Problems ResolutionResolution waste container is Z Motor Home High Waste LevelX Motor Home Test Too Large isTo clean the wash head assembly Chapter 8 Service Service ProceduresTo remove the wash head assembly To replace the wash head assemblyMajor Assemblies AccessoriesSpare Parts HardwareReturning a Washer for Service Returning a Washer for ServiceTo return a Washer for service CERTIFICATE OF DECONTAMINATION EQUIPMENT IN TRANSITEQUIPMENT DECLARATION Suggested decontamination methodsDYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Contact DYNEX TechnologiesContact DYNEX Technologies Index Indexvariable strips, 41 warning labels, 11 Wash Container, 5