Dynex 91000051 manual Specifying Wash Cycle Operations, Creating a New Wash Protocol

Creating a New Wash Protocol

Specifying Wash Cycle Operations

The final step in creating a wash protocol is to specify individual operations within the wash protocol.

An example of a wash protocol is shown in 45. In this example, the dispense wash pins are purged, and then a Dispense and Aspirate are carried out twice on each of the 12 strips (columns) of a 96-well Microtiter® plate. Finally, after all 12 strips have been washed twice, the contents of the Microtiter plate are allowed to soak (equilibrate) for another 600 seconds.

PURGE

↓

MOVE

(2 Cycles)

↓

Purge the dispense pins.

Specify the starting strip and ending strip for each cycle and the number of cycles to be performed.

DISPENSE

(3 Times)

↓

For each strip, perform dispense three times.

ASPIRATE

↓

For each strip, perform one aspiration cycle.

END LOOP

↓

End the cycle.

SOAK

↓

After the two Move cycles have been completed, allow the contents to equilibrate for 600 seconds.

END

End the wash protocol.

Figure 9. Example of a Wash Protocol

The procedures for specifying the steps in this and other wash protocols are described on the following pages.

Opsys MW™ User’s Guide 45