Dynex 91000051 manual Dispense, Soak, Fill, Wash Protocol Operations, Description

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Wash Protocol Operations

	Operation		Description
	Dispense		Dispenses a specified amount of fluid into the wells
	Dispense		Dispenses a specified amount of fluid into the wells
			after aspirating the contents of the wells (i.e.
			wherever a DISPENSE step is added to a protocol,
			the OPSYS MW will automatically aspirate the
			wells before dispensing more fluid in order to
			prevent a well overflow). If a bottom wash is
			specified, the wash head is then lowered to the
			bottom wash position so that fluid will be aspirated
			from the bottom of the wells while fluid is being
			dispensed.
			Note: The dispense height
			for a particular plate type is
			specified during system
			configuration (page 30).
	Soak		The contents of the wells are allowed to equilibrate
			for the specified number of seconds.
	Fill		The wells are filled with a specified amount of fluid.

40	Opsys MW™ User’s Guide

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Contents Please read carefully before using the Opsys MW Opsys MW Microplate WasherUser’s Guide Part No. 91000051, Revision B This manual is published by DYNEX Technologies, IncDYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA Tel. 703 800 288-2354 Fax. 703Warranty and Special Provisions Limited Warranty Page Table of Contents Table of ContentsChapter 2 Installation Chapter 3 ConfigurationChapter 7 Troubleshooting Chapter 8 ServiceIndex Opsys MW User’s GuideAbout this Manual About this ManualThis manual gives you the information needed to This page is intentionally left blank Chapter 1 Introduction DescriptionDescription Programming Wash Head Wash HeadDescription Wash Head Positions Wash Head PositionDescription Dispense HeightWash Fluid WasteDescription Features FeaturesFeatures Specifications Dimensions and WeightPower Requirements SpecificationsInput Ranges StandardsPlate Setup Assay ProgrammingWarning Labels Warning LabelsLabel Description Warning Labels Chapter 2 Installation Unpacking Materials ProvidedUnpacking To unpack the components Hardware Components Hardware ComponentsKeypad. The keypad is used by the operator to select commands and enter wash parameters Connectors at the rear of the Opsys MW system are shown in Figure Figure 3. Rear View of the Opsys MW SystemPositioning the Instrument Positioning the InstrumentInstalling the Wash Head Installing the Wash HeadInstalling the Wash Container To install the Wash ContainerInstalling the Wash Container Installing the Waste Container To install the Waste Container and Foam/Aerosol TrapInstalling the Waste Container Turning on the System Turning on the SystemSETUP PROGRAM 1. Connect the Opsys MW to the laboratory electrical supply outletChapter 3 Configuration The Keypad The Keypad7 8 9 “Cancel” key PurposeScroll keys If you are entering or editing information , aAlphanumeric KeysThe numeral shown on the key is displayed by pressing the key once. If the key is pressed two toSETUP Configure the InstrumentMenus MenusThe SETUP Menu The PROGRAM MenuConfiguring the System Configuring the SystemCommand CLEANSpecifying the Wash Head Specifying Plate Parameters To specify parameters for a plate type Note The default value is displayed. Select SHOW to move the wash head to the default well top position so that the height can be checked. Then, determine the correct value as outlined on page 30. Repeat the procedure as needed Sweep height? SHOW Configuring the System Displaying the Software Version Specifying the Pump Parameters Note Refer to page 74 for additional pump calibration instructionsSelecting Clean Configuring the System Chapter 4 Wash Protocols Wash Protocol Operations Wash Protocol OperationsOperation PurgeDispense SoakFill Creating a New Wash Protocol Specifying the HeaderCreating a New Wash Protocol To create a new wash protocol header 7. Re-enter the password and press ENTER. The display will read Select plate 2 DYNEX1Variable strips? YESNO 9. If YES is selected, the display will read Constant timing? YESNOSelect option MOVE PURGE Specifying Wash Cycle Operations To specify a Purge To specify a Move cycle To specify an Aspiration within a Move Note An Aspirate or Dispense must beTo specify a Dispense within a Move Note A Dispense or Aspirate must be performed within a Move cycleCreating a New Wash Protocol To specify a Soak within a Move To specify a Fill within a Move Note A volume ranging from 50 to 300 microliters can be enteredNote A Fill must be performed within a Move cycle Ending a MOVE Cycle Select option SOAK FILL2. Scroll right to display the next selection. The display will read Select option END LOOP 3. Select END LOOP. The display will readTo specify a Soak outside of a Move To specify the End of the wash protocol Select option SOAK END 2. Select END. The display will read 1 NAME01Modifying a Wash Protocol To modify a wash protocolModifying a Wash Protocol MOVE To modify a wash operation To delete a wash operation To insert a wash operation Copying a Wash Protocol To copy a wash protocolCopying a Wash Protocol 7 NAME04 SETUP PROGRAM Overwrite test? NO YESNO 8. Select YES. The display will readChapter 5 Washing a Plate PreparationTo empty the waste container PreparationTo refill the wash container Washing a Plate To wash a plateWashing a Plate Washing a Plate Chapter 6 Routine Maintenance Routine Maintenance Procedures Daily maintenanceWeekly maintenance Six month maintenanceAs required maintenance Cleaning and Decontamination To clean the systemCleaning and Decontamination To decontaminate the systemCleaning the Containers To empty and clean the waste containerCleaning the Containers Replacing the Tubing To replace the dispense tubingReplacing the Tubing To replace the vacuum tubing To replace the waste tubing Pump Calibration Calibration ProtocolPump Calibration 8. If the dispensed volume is too high, decrease the pump speed using the SETUP/CAL function refer to page Pump Calibration Chapter 7 Troubleshooting Operational Problems Operational ProblemsSymptom ResolutionOperational Problems waste container is High Waste Level X Motor HomeZ Motor Home Test Too Large isChapter 8 Service Service Procedures To remove the wash head assemblyTo clean the wash head assembly To replace the wash head assemblyAccessories Spare PartsMajor Assemblies HardwareReturning a Washer for Service To return a Washer for serviceReturning a Washer for Service EQUIPMENT IN TRANSIT EQUIPMENT DECLARATIONCERTIFICATE OF DECONTAMINATION Suggested decontamination methodsContact DYNEX Technologies Contact DYNEX TechnologiesDYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151 USA Index Indexvariable strips, 41 warning labels, 11 Wash Container, 5