Troubleshooting (continued)

Polarization contrast cannot be adjusted.

Bring the polarizer and analyzer into cross position until they reach maximum darkness (without specimen).

Phase contrast

Phase contrast cannot be adjusted.

The specimen is too thick, too thin, or too brightly stained.

Refractive indexes of the mounting medium and specimen are identical, so that there is no phase jump.

The cover glass is not placed uniformly.

Check that the correct light ring is positioned.

Check the centering of the light rings.

Check the condenser centering.

Open the aperture diaphragm completely.

Specimen Stage

Positioning range of the stage in the x-direc- tion decreases after working a long time.

Move the mechanical stage with coaxial drive all the way to the left.

Manually press the screw that holds the mechanical stage even farther to the left, as far as it will go. Then move the mechanical stage with coaxial drive all the way to the right.

Manually press the screw that holds the mechanical stage even farther to the right, as far as it will go.

Leica DM750

Manual

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Leica DM750 manual Phase contrast