UVPS 81-0112-01 Rev C manual Applications

Page 8

UV CROSSLINKERS

UVP

5.APPLICATIONS

The Crosslinker is a multi-purpose ultraviolet exposure instrument for use in the laboratory. A wide variety of applications for ultraviolet radiation exist in the laboratory.

A.UV crosslinking of DNA and RNA by covalently binding nucleic acids to transfer membranes nitrocellulose, nylon or nylon-reinforced nitrocellulose membranes after Northern, Southern, slot or dot blotting.

The PRESET ULTRAVIOLET EXPOSURE SETTING is factory set to a UV dose of 120,000 microjoules per cm² for this laboratory purpose. This setting has been found to be the optimal dose for DNA retention and hybridization-signal sensitivity.

B.Nicking ethidium-bromide stained DNA in Agarose Gels

C.Gene Mapping for creating cleavage-inhibiting thymine dimers

D.Testing RecA function

E.Ultraviolet Sterilization

F.Elimination of PCR contamination

G.UV Curing

H.Fluorescence of materials

Further Applications can be achieved with the Crosslinkers by changing the ultraviolet wavelength (see Section 7 Changing the wavelength).

- 6 -

Image 8
Contents Ultraviolet Crosslinkers Contents Introduction Important Safety InformationDescription and Specifications Features ElectricalCL-1000 Shortwave Crosslinker CL-1000L Longwave CrosslinkerOperation UV Crosslinkers UVP UV Crosslinkers UVP Applications MAINTENANCE, Care and Cleaning of the Crosslinker UV Wavelength Calibration Procedure Changing the UV WavelengthUV Crosslinkers UVP Crosslinker Calibration Procedure Equipment necessary Voltage Range for U4 Range for U5 Replacement Parts and Servicing Procedures Operations with TOP Cover RemovedOperations With Top Cover Removed Removal of top cover General Servicing ProceduresReplacement of tube holders Replacement of starters Replacement of starter holderReplacement of main switch Replacement of fuse holdersReplacement of main connector Replacement of membrane panel Replacement of UV sensorReplacement of PCB Replacement of reflector General Items Changing fuses Changing plastic feetSchematic Wiring Diagram Corporate Headquarters UVP, Inc