Leica B523 manual Saving and transferring images, Finishing your imaging, Scale bars

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3.6.2Scale bars

The Studio Lite software does not provide means to insert scale bars. They can be added later on in any image editing software such as Adobe Photoshop. Ready-made

scale bars for the full resolution images are available at http://www.miu.helsinki.fi/instruments/LeicaDMLB/scale_pictures.htm (in Photoshop format). The page also contains the scaling information (i.e. how many micrometers each pixel represents), so you can draw the scale bars yourself.

4 Saving and transferring images

Save images using the TIFF format to prevent image data loss. The default save format is JPEG, which discards color information invisible to human eye, thus decreasing image quality and making quantitative analysis unreliable.

None of the image formats save the metadata i.e. objectives, exposure times, microscope model etc. You should always write down this information, as you will need it when you want to publish your images.

Images can be saved through network in a temporary disk space: Images –folder in

Snap Server (Mcbserver1). The network drive is mounted as drive I: Images on Mcbserver1 (Figure 20). Note that this disk space is occasionally emptied on Mondays without announcement, so do not use it for long-term storage.

At your own computer, connect to Mcbserver1 as follows:

Windows - by writing in the Explorer location bar: \\mcbserver1\images

Mac - by connecting to the server in Finder using the address: smb://mcbserver1/images

The password needed in both cases is provided by the MIU staff.

Figure 20. Mcbserver1 (Snap server) icon

Files can also be saved locally and transferred to a USB stick. There is a USB extension cable next to the keyboard. Remember to delete locally stored images once you have transferred them to your own computer.

5 Finishing your imaging

When you have finished your imaging session you should:

Log off the computer

Shut down the computer if you are the last user of the day

Turn off the microscope (Figure 1 on page 1) and clean it if necessary

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Contents 2008 Saving and Transferring Images Settings for transmitted light microscopy General user informationTurning on the microscope XY-stagePlacing the sample ObjectivesFocusing Auxiliary condenser lens switchEye pieces oculars Dual viewing attachmentDual viewing attachment Koehler illumination InstructionInstruction Polarization contrast FiltersPage Accessing the computer View Finder application Adjustments before image acquisitionCapture resolution Image acquisitionLevels adjustment Exposure ModeWhite balance Over/Underexposure Focus toolAveraging Post-imaging adjustments Capturing the imageViewing images Cropping the imageFinishing your imaging Saving and transferring imagesScale bars Troubleshooting Computer login fails