Leica B523 manual Polarization contrast, Filters

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2.9 Polarization contrast

Figure 10. Analyzer for polarization contrast imaging

Figure 11. Polarizer

For polarization contrast microscopy, an analyzer (a polarizing filter) is inserted in the light path by pushing it all the way in (Figure 10), and another polarizing filter (polarizer) is placed labeled side upwards in a holder below the condenser (Figure 11). The round polarizer is on the shelf above the microscope.

To cross the polarizing filters, remove your sample and turn the round polarizer until the maximum extinction position is reached, i.e. the view in the oculars is as dark as possible.

2.10 Filters

On the right side of the microscope, there are controls for three filters (Figure 12):

DLF: a blue daylight filter which was mainly used for photography with daylight film

NI6: a grey (neutral density) filter that attenuates light to 6.25 % transmission without interfering color

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Contents 2008 Saving and Transferring Images Turning on the microscope Settings for transmitted light microscopyGeneral user information XY-stagePlacing the sample ObjectivesFocusing Auxiliary condenser lens switchEye pieces oculars Dual viewing attachment Dual viewing attachment Koehler illumination InstructionInstruction Polarization contrast FiltersPage Capture resolution Accessing the computer View Finder applicationAdjustments before image acquisition Image acquisitionLevels adjustment Exposure ModeWhite balance Over/Underexposure Focus toolAveraging Viewing images Post-imaging adjustmentsCapturing the image Cropping the imageFinishing your imaging Saving and transferring imagesScale bars Troubleshooting Computer login fails