Leica B523 manual 2008

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Leica DB LB Research microscope and Studo Lite Imaging software

Room B523

User Guide

Molecular Imaging Unit

University of Helsinki

www.miu.helsinki.fi

9.4.2008

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Contents 2008 Saving and Transferring Images General user information Settings for transmitted light microscopyTurning on the microscope XY-stageObjectives Placing the sampleAuxiliary condenser lens switch FocusingDual viewing attachment Eye pieces ocularsDual viewing attachment Instruction Koehler illuminationInstruction Filters Polarization contrastPage Adjustments before image acquisition Accessing the computer View Finder applicationCapture resolution Image acquisitionLevels adjustment Exposure ModeWhite balance Over/Underexposure Focus toolAveraging Capturing the image Post-imaging adjustmentsViewing images Cropping the imageFinishing your imaging Saving and transferring imagesScale bars Troubleshooting Computer login fails