Leica B523 manual Placing the sample, Objectives

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movement in y-direction (back and forth), lower in x-direction (side to side). If you need to rotate the stage to get the image in any wanted angle, open the rotation lock by unscrewing it and then rotating the stage by hand (circle in Figure 2). Re-screw the lock after use!

Figure 2. Adjustment wheels for stage control (arrows) and rotation lock (circle)

2.3 Placing the sample

Place your sample on the specimen stage by manually widening and releasing the spring-operated object holder (Figure 3).

2.4 Objectives

Select an appropriate objective (Table 1) by manually turning the black rim of the objective revolver (Figure 3).

Figure 3. Objective revolver (red arrow) and sample holder

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Contents 2008 Saving and Transferring Images Settings for transmitted light microscopy General user informationTurning on the microscope XY-stagePlacing the sample ObjectivesFocusing Auxiliary condenser lens switchEye pieces oculars Dual viewing attachmentDual viewing attachment Koehler illumination InstructionInstruction Polarization contrast FiltersPage Accessing the computer View Finder application Adjustments before image acquisitionCapture resolution Image acquisitionLevels adjustment Exposure ModeWhite balance Over/Underexposure Focus toolAveraging Post-imaging adjustments Capturing the imageViewing images Cropping the imageFinishing your imaging Saving and transferring imagesScale bars Troubleshooting Computer login fails