Image acquisition, Capture resolution, Sensitivity

3 Image acquisition

3.1 Accessing the computer

If the computer is turned off, push the power button to turn it on. When the login screen appears, log in using your hyad login account. Check that the domain is set to LTDK.

3.2 View Finder application

A 10-bit Olympus DP50 color CCD camera is attached on the top of the microscope. It is controlled by a computer and Studio Lite software.

To capture an image, open the Pixera Corporation Studio Lite program by double- clicking its icon on the desktop (Figure 14). Then, open up View Finder by clicking the camera button in the menu at the top.

Figure 14. Starting the Studio Lite and View Finder applications

Figure 15. Adjustments in View finder window

∙Press ‘Live image’ button (green triangle in Figure 15) in the View Finder window. A live image of your sample will be shown on the screen. The speed of the image refresh can be adjusted by selecting Fast, Zoom, or Full in the main menu under View Finder Mode. It is easier to focus and move the sample with the Fast setting. For fine adjustment of the focal plane, use Zoom.

3.3Adjustments before image acquisition

3.3.1Capture resolution

There are three resolutions (640x480, 1392x1040, and 2776x2074) to choose from (Figure 15). Use higher resolution for more details, if the bigger file size does not matter. The highest resolution is slower to acquire than the other two. Resolution selection does not affect the live view image.

3.3.2Sensitivity

In the Sensitivity box, select ISO values from 50 to 400. Higher value shortens the exposure time but increases background noise and decreases image quality. In digital imaging, ISO 100 is the level of intensity read from the sensor. Everything else is