Leica B523 manual Troubleshooting

Page 17

Cover the microscope with the red hood

6 Troubleshooting

6.1There is no light

Microscope is not turned on (see Section 2.1)

Light intensity set too low (see 2.1 on page 1)

Do you see any light in the lamp chamber? If not, the halogen bulb may need to be changed - contact MIU

6.2Light is too dark

Check:

whether the polarizer or other filters are on the light path (Section 2.9 on page 8)

that aperture diaphragm is not too closed (Section 2.8 on page 6)

6.3Problems with focus or picture clarity

Check that:

no filters are on the light pathway

Koehler illumination is correctly done (Section 2.8 on page 6)

objective is not dirty

oculars are not dirty

oculars are correctly adjusted (Section 2.6)

If image quality does not satisfy you, you should take your images with AxioPlan microscope, which has better optics and camera

6.4 View is partial

Check that:

none of the filters are partially on the light path

the polarizer is not partially on the light path

the objective is correctly in place

the field diaphragm is not too closed

6.5Colors have red/yellow tint

Check the white balance

For other color errors, check the color filters (Section 2.10)

6.6Ocular view is not at the same focus as the camera view

Check ocular adjustment (Section 2.6)

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Contents 2008 Saving and Transferring Images General user information Settings for transmitted light microscopyTurning on the microscope XY-stageObjectives Placing the sampleAuxiliary condenser lens switch FocusingDual viewing attachment Eye pieces ocularsDual viewing attachment Instruction Koehler illuminationInstruction Filters Polarization contrastPage Adjustments before image acquisition Accessing the computer View Finder applicationCapture resolution Image acquisitionWhite balance Exposure ModeLevels adjustment Averaging Focus toolOver/Underexposure Capturing the image Post-imaging adjustmentsViewing images Cropping the imageScale bars Saving and transferring imagesFinishing your imaging Troubleshooting Computer login fails