Battery Replacement, Calibration Check Chart

Care and Maintenance

Battery Replacement

If the battery does not hold a sufficient charge for proper operation of the pipettor, follow these steps for replacement of the battery.

1.Switch the unit "OFF".

2.Remove the top two screws on the back of the pipettor and remove the battery cover.

3.Carefully remove the battery by lifting it straight out of the holder.

4.Install the new battery by pressing the positive (+) end against the contact spring at the bottom of the holder. Guide the negative (-) end carefully into position.

5.Replace the cover and the screws. Do not overtighten the screws.

6.Dispose of battery according to local requirements or regulations.

Calibration Check (Mode d1, 1:5, 5mL dilution)

Genuine 3M Pipettor Tips are strongly recommended for verifying pipettor performance. Perform a single-sample measurement following a change of any removable parts. Perform a 4-sample test monthly–or more frequently, depending on use. Perform a 10-sample test quarterly. Record all results and maintain records. Use the procedure indicated in “Standard Methods for the Examination of Dairy Products.”

1.The pipettor, pipettor tips and test liquid should all be allowed to adjust to room temperature (19°C-25°C) for one day before beginning the calibration check.

Note the temperature before starting.

2.Double-rinse the pipettor tip by aspirating water to the 5.0 mL level (4.0mL + 1.0mL) and then expelling it.

3.Place a 50mL or smaller vessel containing 20-25g of distilled water onto an analytical balance. Note its weight. Re-tare balance to 0 (zero).

4.Aspirate 4.0mL of water from the vessel on the analytical balance. Note its weight. Re-tare balance to 0.

5.Aspirate an additional 1.00 mL from the vessel on the analytical balance. Note its weight. Re-tare balance to 0.

6.Expel the 5.0mL of water back into the vessel on the analytical balance. Note its weight.

7.Repeat steps 4 - 6 as desired.

8.Calculate the average weight of the 4.0mL aspirations. Repeat this calculation for the 1.00 mL and 5.0mL measurements.

9.Refer to Table 1, page 26. Find the temperature closest to the temperature you recorded during step 1. Confirm that each average weight falls within the corresponding weight tolerance range for that temperature.

Page 13 of 15 - June 2001

Care and Maintenance

Table 1

Calibration Check Chart

1:5 Dilution	4.00 mL	1.00 mL	5.00 mL
Test Volume>	4.00 mL	1.00 mL	5.00 mL
Volume (mL)
Tolerance Range >	3.968-4.032 mL	0.979-1.021 mL	4.945- 5.055 mL

	Weight (g)	Weight (g)	Weight (g)
Temperature	Tolerance Range*	Tolerance Range*	Tolerance Range*

19°C	3.9585 - 4.0207g	0.9767 - 1.0181g	4.9332 - 5.0409g


20°C	3.9577 - 4.0199g	0.9765 - 1.0179g	4.9322 - 5.0399g


21°C	3.9569 - 4.0191g	0.9763 - 1.0177g	4.9312 - 5.0389g


22°C	3.9557 - 4.0179g	0.9760 - 1.0174g	4.9297 - 5.0374g

23°C	3.9549 - 4.0171g	0.9758 - 1.0172g	4.9287 - 5.0364g


24°C	3.9542 - 4.0163g	0.9756 - 1.0170g	4.9278 - 5.0354g


25°C	3.9530 - 4.0151g	0.9753 - 1.0167g	4.9263 - 5.0339g


26°C	3.9522 - 4.0139g	0.9751 - 1.0164g	4.9253 - 5.0324g


27°C	3.9510 - 4.0131g	0.9748 - 1.0162g	4.9238 - 5.0314g

28°C	3.9498 - 4.0119g	0.9745 - 1.0159g	4.9224 - 5.0299g


29°C	3.9487 - 4.0107g	0.9742 - 1.0156g	4.9209 - 5.0283g


30°C	3.9475 - 4.0099g	0.9739 - 1.0154g	4.9194 - 5.0273g

* Weights adjusted for temperature and pressure effects using the “z factor”.

Care and Maintenance