Repeat Dispensing 1-5 repetitions of 1mL

1.Press repeatedly until the display reads:

2.Press to enter selection.

3.Attach a pipette tip to the tip cone.

The pipettor is now ready to begin the 1:10 dilution (1mL total volume). The display indicates that the pipettor is ready to aspirate 0.90mL.

4.Place the tip in the diluent and press the

Start Button. The unit will aspirate 0.90mL.

The following display indicates that the unit is ready to aspirate 0.10mL.

5. Place the tip in the sample solution and press the Start Button to aspirate 0.10mL.

The next display indicates that the unit is ready to dispense the total volume (1mL) with blowout.

6.Position the pipette tip to dispense and press the Start Button. The unit will

dispense the full amount from the tip.

The display now indicates that the unit is ready to repeat the dilution sequence, beginning with the 0.90mL aspiration.

Page 7 of 15 - June 2001

Repeat Dispensing (1-5 repetitions of 1mL)

1.Press repeatedly until the display reads:

2.Press or to select a new number of 1ml repetitions (1-5). In this case 3.

3.Press to enter selection.

4.Attach a pipette tip to the tip cone.

The display indicates that the pipettor is ready to aspirate 3.00mL.

5. Place the tip in the liquid and press the Start Button. The unit will aspirate 3.00mL.

The following display indicates that the unit is ready to dispense the first of the three 1.00mL aliquots:

6. Position the pipette tip to dispense and

press the Start Button. The unit will dispense the first 1.00mL aliquot.

The following display indicates that unit is ready to dispense the second of the three 1.00mL aliquots: