4. Operation

4.3Conversion table T% to AU and OD

4.4 Start-up

4.5Stabilization time

T%

AU

OD

OD

 

 

(3 mm cell)

(10 mm cell)

 

 

 

 

1

2.000

6.667

2.000

5

1.301

4.337

1.301

10

1.000

3.333

1.000

15

0.824

2.747

0.824

20

0.699

2.330

0.699

25

0.602

2.007

0.602

30

0.523

1.743

0.523

32

0.500

1.667

0.500

35

0.456

1.520

0.456

40

0.398

1.327

0.398

45

0.347

1.157

0.347

50

0.301

1.003

0.301

55

0.260

0.867

0.260

60

0.222

0.740

0.222

63

0.200

0.667

0.200

65

0.186

0.620

0.186

70

0.155

0.517

0.155

75

0.125

0.417

0.125

79

0.100

0.333

0.100

80

0.097

0.323

0.097

85

0.070

0.233

0.070

89

0.050

0.167

0.050

90

0.046

0.153

0.046

95

0.022

0.073

0.022

95.5

0.020

0.067

0.020

97.7

0.010

0.033

0.010

Note: Always ensure that all liquid passing through the flow cell are degassed to prevent any air bubble formation in the cell. Liquids must be filtered to remove any particulate material and prevent any blockage.

1.Check that the monitor is correctly installed and that the sample cell is filled with the appropriate eluent. The reference cell may be left closed with air in the cell unless the UV-1 is used with eluents showing appreciable UV-absorption. In this case the reference cell should be filled with eluent.

2.If the instrument has been switched off, turn on the mains switch (Fig. 5:16) and refer to Section 4.5, to restabilize the instrument.

3.Check that the appropriate filter and converter or aperture are in place and fully inserted.

At normal laboratory temperatures the UV-1 requires 2 hours to stabilize sufficiently. When the UV- 1 is in constant use, it is recommended that it remains switched on. UV- 1 can be switched off when not in use for periods of one week or more.

For coldroom operation below 10 °C, install UV-1 in the coldroom at the desired running temperature at least 12 hours before the start of a run. This is necessary to allow the instrument housing to equilibrate to the temperature of the coldroom. Once equilibration has taken place, the stabilisation times given for normal temperatures are valid.

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