4. Operation
4.6Basic operating procedure -
AU
4.7Basic operating procedure - Transmission (%T)
4.8 Shut down
1.Switch AU/ %T to AU (Fig. 5:13).
2.Set the range selector (Fig. 5:12) to SHORT and zero the recorder with the recorder zero control.
3.Set the range selector to 2 and adjust the recorder baseline with the baseline adjust (Fig. 5:14).
4.Set the range selector to the appropriate range and readjust the recorder baseline with the baseline adjust (Fig. 5:14). Only minor adjustment should be necessary.
1.Switch AU to %T (Fig. 5:13).
2.Set the range selector (Fig. 5:12) to SHORT and zero the recorder.
3.Turn the baseline adjust (Fig. 5:14) fully clockwise.
4.Set the range selector so that the recorder gives a deflection just greater than 100%.
5. Adjust baseline to bring the pen back to 100%.
6.Exchange the converter for the shutter and zero the recorder with the recorder zero. This response will correspond to 0% transmission.
7.Replace the shutter with the converter. This response will correspond to 100% transmission. Slight adjustment of baseline may be required to obtain the response obtained in Step 5.
1.On completion of the chromatographic run, flush the flow cell either with pure solvent or the buffer used in the chromatographic run. To prevent the deposition of salts from aqueous buffers, flush the cell with distilled water after use, if necessary after disconnecting the column.
Note: Never allow aqueous buffers to dry out in the cell. Either continue to flush through with buffer or leave the cell filled with distilled water.
2.Leave the
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