New Objective with Low Chromatic Aberration Delivers World-Leading Imaging Performance.

NEW Low Chromatic Aberration Objective

Best Reliability for Colocalization Analysis

A new high NA oil-immersion objective minimizes chromatic aberration in the 405–650 nm region for enhanced imaging performance and image resolution at 405 nm. Delivers a high degree of correction for both lateral and axial chromatic aberration, for acquisition of 2D and 3D images with excellent and reliable accuracy, and improved colocalization analysis. The objective also compensates for chromatic aberration in the near infrared up to 850 nm.

Lateral and Axial Chromatic Aberration

Small Degree of

Large Degree of

Chromatic Aberration

Chromatic Aberration

Objective

Axial chromatic

aberration

(Z direction).Lateral chromatic aberration (X-Y

direction at FN6).

Performance Comparison of PLAPON 60xOSC and UPLSAPO 60xO

 

PLAPON60xOSC

UPLSAPO60xO

Axial chromatic

 

 

aberration (Z direction)

 

 

Compared for PSF fluorescent

Approx.

 

beads (405 nm, 633 nm).

 

0 µm

Approx.

 

 

 

 

 

0.5 µm

Lateral chromatic

 

 

aberration (X-Y direction)

 

 

Compared for PSF fluorescent

 

 

beads (405 nm, 488 nm, 633

 

 

nm).

Approx. 0.1 µm

Approx. 0.2 µm

3D image

 

 

Tubulin in Ptk2 cells labeled with

 

 

two colors (405 nm, 635 nm) and

 

 

compared.

 

 

 

 

 

Low Chromatic

PLAPON60xOSC

Aberration Objective

Magnification: 60x

 

NA: 1.4 (oil immersion)

 

W.D.: 0.12 mm

 

Chromatic aberration compensation range: 405–650 nm

 

Optical data provided for each objective.

Chromatic Aberration Comparison for PLAPON 60xOSC and UPLSAPO 60xO

 

 

 

Lateral Chromatic Aberration

 

 

 

0.5

 

 

 

 

 

 

 

 

UPLSAPO60xO

 

 

 

(µm)

 

 

 

 

 

 

Focal plane

0.0

 

 

 

 

 

 

 

PLAPON 60xOSC

 

 

 

 

-0.5

 

 

 

 

 

 

400

450

500

550

600

650

Wavelength (nm)

*Chromatic aberration values are design values and are not guaranteed values.

Improved Flatness and Resolution at 405 nm

Better flatness reduces the number of images for tiling.

Flatness Comparison Image at 1x Zoom

PLAPON60xOSCUPLSAPO60xO

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Olympus FV1000 manual NEW Low Chromatic Aberration Objective, Best Reliability for Colocalization Analysis