Olympus FV1000 Main Specifications, Objectives for BX2, Using U-UCD8A-2, IX2-LWUCDA2 and U-DICTS

Main Specifications

		Spectral Version	Filter Version
Laser Light	Ultraviolet/Visible Light Laser	LD lasers: 405 nm: 50 mW, 440 nm: 25 mW, 473 nm: 15 mW, 559 nm: 15 mW, 635 nm, 20 mW
		Multi-line Ar laser (458 nm, 488 nm, 515 nm, Total 30 mW), HeNe(G) laser (543 nm, 1 mW)
	AOTF Laser Combiner	Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control
		Continuously variable (0.1%–100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region
	Fiber	Broadband type (400 nm–650 nm)
Scanning and	Scanner Module	Standard 3 laser ports, VIS – UV – IR
Detection		Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y)
		Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser
	Detector Module	Standard 3 confocal Channels (3 photomultiplier detectors)	Standard 3 confocal Channels (3 photomultiplier detectors)
		Additional optional output port light path available for optional units	Additional optional output port light path available for optional units
		6 position beamsplitter turrets with CH1 and CH2	6 position beamsplitter turrets with CH1 and CH2
		CH1 and CH2 equipped with independent grating and slit for fast and	CH1 to CH3 each with 6 position barrier filter turret
		flexible spectral detection	(High performance filters)
		Selectable wavelength bandwidth: 1–100 nm
		Wavelength resolution: 2 nm
		Wavelength switching speed: 100 nm/msec
		CH3 with 6 position barrier filter turret
	Filters	High performance sputtered filters, dichromatic mirrors and barrier filters
	Scanning Method	2 galvanometer scanning mirrors
	Scanning Modes	Scanning speed: 512 x 512 (1.1 sec., 1.6 sec., 2.7 sec., 3.3 sec., 3.9 sec., 5.9 sec., 11.3 sec., 27.4 sec., 54.0 sec.)
		256 x 256 bidirectional scanning (0.064 sec., 0.129 sec.)
		X,Y,T,Z,λ	X,Y,T,Z
		Line scanning: Straight line with free orientation, free line, Point scanning	Line scanning: Straight line with free orientation, free line, Point scanning
	Photo Detection Method	2 detection modes: Analog integration and hybrid photon counting
	Pinhole	Single motorized pinhole	Single motorized pinhole
		pinhole diameter ø50–300 µm (1 µm step)	pinhole diameter ø50–800 µm (1 µm step)
	Field Number (NA)	18
	Optical Zoom	1x–50x in 0.1x increment
	Z-drive	Integrated motorized focus module of the microscope, minimum increment 0.01 µm or 10 nm
	Transmitted Light	Module with integrated external transmitted light photomultiplier detector and 100 W Halogen lamp, motorized switching, fiber adaptation to microscope
	Detector unit	frame
Microscope	Motorized Microscope	Inverted IX81, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI
	Fluorescence Illumination	External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit
	Unit	Motorized switching between LSM light path and fluorescence illumination
System Control	PC	PC-AT compatible, OS: Windows XP Professional (English version), Windows Vista (English version), Memory: 2.0 GB or larger, CPU:Core2Duo 3.0 GHz,
		Hard disk: 500 GB or larger, Media: DVD Super Multi Drive, FV1000 Special I/F board (built-in PC), Graphic board: conformity with Open GL
	Power Supply Unit	Galvo control boards, scanning mirrors and gratings, Real time controller	Galvo control boards, scanning mirrors
	Display	SXGA 1280X1024, dual 19 inch (or larger) monitors or WQUXGA 2560 x 1600, 29.8 inch monitor
Optional Unit	SIM Scanner	2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port, Fiber introduction of near UV diode laser or visible light laser,
		Optional: 2nd AOTF laser combiner
	TIRFM Unit	Available laser: 405–633 nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives
	4th CH Detector	Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path
	Fiber Port for Fluorescence	Output port equipped with FC fiber connector (compatible fiber core 100–125 µm)
Software
Image Acquisition		Normal scan: 64 x 64, 128 x 128, 256 x 256, 320 x 320, 512 x 512, 640 x 640, 800 x 800, 1024 x 1024, 1600 x 1600, 2048 x 2048, 4096 x 4096
		Clip rectangle scan ,Clip ellipse scan ,Polygon clip scan,line scan ,free line scan,Point scan, Real-time image
		2-dimension: XY, XZ, XT and Xλ
		3-dimension: XYZ, XYT, XYλ , XZT, XTλ and XZλ
		4-dimension: XYZT, XZTλ and XYTλ
		5−dimension: XYZTλ
Programmable Scan Controller		Time Controller function
2D Image Display		Each image display: Single-channel side-by-side, merge, cropping, live tiling, live tile, series (Z/T/λ ),
		LUT: individual color setting, pseudo-color, comment: graphic and text input
3D Visualization and Observation		Interactive volume rendering: volume rendering display, projection display, animation displayed (save as OIF, AVI or MOV format)
		Free orientation of cross section display
		3D animation (maximum intensity projection method, SUM method)
		3D and 2D sequential operation function
Image Format		OIB/ OIF image format
		8/ 16 bit gray scale/index color, 24/ 32/ 48 bit color,
		JPEG/ BMP/ TIFF/ AVI/ MOV image functions
		Olympus multi-tif format
Spectral Unmixing		2 Fluorescence spectral unmixing modes (normal and blind mode)
Image Processing		Filter type: Sharpen, Average, DIC Sobel, Median, Shading, Laplacian
		Calculations: inter-image, mathematical and logical, DIC background leveling
Image Analysis		Fluorescence intensity, area and perimeter measurement, time-lapse measurement
Statistical Processing		2D data histogram display, colocalization
Optional Software		Review station software, Off-line FLUOVIEW software for date analysis.
		Motorized stage control software, Diffusion measurement package, Multi stimulation software, Multi area time-lapse software

Objectives for BX2 and IX2

(using U-UCD8A-2, IX2-LWUCDA2 and U-DICTS)

		W.D.	Cover glass		Correction	Condenser for BX2	Condenser for IX2	U-DICTS
Description	NA		thickness	Immersion		U-UCD8A-2	IX2-LWUCDA2
		(mm)			ring			position
			(mm)			optical element	optical element
UPLSAPO4X	0.16	13	—
UPLSAPO10X2	0.40	3.1	0.17			U-DIC10	IX2-DIC10	normal
UPLSAPO20X	0.75	0.6	0.17			U-DIC20	IX2-DIC20	normal
UPLSAPO20XO	0.85	0.17	—	Oil		U-DIC20	IX2-DIC20	normal
UPLSAPO40X2	0.95	0.18	0.11-0.23		_	U-DIC40	IX2-DIC40	normal
UPLSAPO60XO	1.35	0.15	0.17	Oil		U-DIC60	IX2-DIC60	BFP1
UPLSAPO60XW	1.20	0.28	0.13-0.21	Water	_	U-DIC60	IX2-DIC60	normal
UPLSAPO100XO	1.40	0.12	0.17	Oil		U-DIC100	IX2-DIC100	normal
PLAPON60XO	1.42	0.15	0.17	Oil		U-DIC60	IX2-DIC60	BFP1
PLAPON60XOSC	1.40	0.12	0.17	Oil		U-DIC60	IX2-DIC60	BFP1
UPLFLN40XO	1.30	0.2	0.17	Oil		U-DIC40	IX2-DIC40	BFP1
APON60XOTIRF	1.49	0.1	0.13-0.19	Oil	_	U-DIC60	IX2-DIC60	BFP1
UAPON100XOTIRF	1.49	0.1	0.13-0.19	Oil	_	U-DIC100	IX2-DIC100	normal
UAPON150XOTIRF	1.45	0.08	0.13-0.19	Oil	_	U-DIC100	IX2-DIC100	normal
Apo100XOHR	1.65	0.1	0.15	Oil		U-DIC100	IX2-DIC100	normal

Objectives for fixed stage upright microscope

(using WI-UCD, WI-DICTHRA2)

Objectives	NA	W.D. (mm)	DIC prism	Revolving
				nosepiece
MPLN5X	0.10	20.00	–	WI-SSNP,
				WI-SRE3
UMPLFLN10XW	0.30	3.50	WI-DIC10HR	WI-SSNP,
				WI-SRE3
UMPLFLN20XW	0.50	3.50	WI-DIC20HR	WI-SSNP,
				WI-SRE3
LUMPLFLN40XW	0.80	3.30	WI-DIC40HR	WI-SSNP,
				WI-SRE3
LUMPLFLN60XW	1.00	2.00	WI-DIC60HR	WI-SSNP,
				WI-SRE3
LUMFLN60XW	1.10	1.5	WI-DIC60HR	WI-SSNP,
				WI-SRE3
XLUMPLFLN20XW	1.00 *	2.0	WI-DICXLU20HR	WI-SNPXLU2

*Note: These conditions are not met in confocal microscopy

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