This optional software module enables data acquisition and analysis to investigate the molecular interaction
and concentrations by calculating the diffusion coefficients of molecules within the cell.
Diverse analysis methods (RICS/ccRICS, point FCS/point FCCS and FRAP) cover a wide range of molecular
sizes and speeds.
Diffusion Measurement Package
105
105
125
Pixels
Pixels
125
130
130
0
0.5
1
1.5

p

oint FCS

RICS

FRAP

> 1
00
Capable range
of measurement
~ 1
00
1 ~ 1
00
<
0
.
1
<
0
.
01
<<
.
Small molecules
in
so
l
u
ti
o
n
Proteins
in
so
l
u
ti
o
n
Diffusion of
p
roteins
in
ce
ll
Molecular com
p
le
x
formation
,
a
gg
re
g
ation
Protein
traffickin
g
Lateral diffusion
in
ce
ll m
e
m
b
r
a
n
e

RICS—Raster Imaging Correlation Spectroscopy

Raster image correlation spectroscopy (RICS) is a new method for analyzing the diffusion

and binding dynamics of molecules in an entire, single image. RICS uses a spatial

correlation algorithm to calculate diffusion coefficients and the number of molecules in

specified regions.

Cross correlation RICS (ccRICS) characterizes molecular interactions using fluorescent-

labeled molecules in two colors.

FRAP Analysis

The Axelrod analytical algorithm is installed as a FRAP analysis method. The algorithm is used to calculate diffusion coefficients and the

proportions of diffusing molecules.

point FCS—Point scan Fluorescence Correlation Spectroscopy

point scan fluorescence correlation spectroscopy (point FCS) analyzes intensity

fluctuations caused by diffusion or binding/unbinding interactions of a protein complex.

point FCS uses an auto correlation function to carry out operations on fluorescence

signals obtained by continuous scanning of a single pixel on the screen.

point scan fluorescence cross-correlation spectroscopy (point FCCS) analyzes the

fluctuation of fluorescent-labeled molecules in two colors. The coincidence of fluctuations

occurring in two detection channels shows that the two proteins are part of the same

complex.

point FCS and point FCCS can now be performed with a standard detector, eliminating

the need for a special high-sensitivity detector.

Analytical methods

according to molecule

diffusion speeds

15