Olympus FV1000 Multi-Dimensional Time-Lapse, Significantly Improved Long Time-Lapse Throughput

	P2	Multi-Dimensional Time-Lapse
P1		P3 The FV1000 can be used for ideal multi-dimensional time-lapse imaging during confocal observation,
		using multi-area time-lapse software to control the motorized XY stage and focus compensation.

P4

P5

Significantly Improved Long Time-Lapse Throughput

Equipped with motorized XY stage for repeated image acquisition from multiple points scattered across a wide area. The system efficiently analyzes changes over time of cells in several different areas capturing, large amounts of data during a single experiment to increase the efficiency of experiments. Microplates can be used to run parallel experiments, which significantly improves throughput for experiments that require long-term observation.

Supports repeated image acquisition from multiple areas in a single microplate well.

Multi-Point Time-Lapse Software

Point 2

Point 3

Point 1

Focal Plane 4

Focal Plane 3

Focal Plane 2

Focal Plane 1

Point 4

Point 5

Point 6

Focal Drift Compensation for Long Time-Lapse Imaging

The IX81-ZDC Zero Drift Compensation system corrects loss of focus caused by temperature changes around the microscope and other factors during long time-lapse observation. The thermal drift compensation eliminates the need to take images at several Z planes, minimizing live cell exposure to irradiation.

			Offset
Objective	IR Laser for focal	Baseline focal plane
focal	plane detection
plane

Set target	Over time, the	Laser detects	Immediately
observation	objective focal	the glass	returns to initial
plane	plane drifts from	surface before	offset plane,	ZDC
as offset.	the observation	imaging.	for focal drift
	plane.		compensation.

Scanning unit

Maintain Cell Activity Over A Long Period

Proprietary CO2 incubator control keeps the environment inside the tissue culture dish completely stable. The environment is precisely maintained at 37°C with 90% humidity and 5% CO2 concentration.

0 s 1000 s 2000 s 3000 s 4000 s 5000 s 6000 s 7000 s

Human lymphoblast cells TK6

Courtesy of: Masamitsu Honma, Dir.

Biological Safety Research Center Div. of Genetics and Mutagenesis I, National Institute of Health Sciences

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