Olympus FV1000 manual Spectral Based Detection, Filter Based Detection

Two Versions of Light Detection System that Set New Standards for Optical Performance.

Spectral Based Detection

Flexibility and High Sensitivity

Spectral detection using gratings for 2 nm wavelength resolution and image acquisition matched to fluorescence wavelength peaks. User adjustable bandwidth of emission spectrum for acquiring bright images with minimal cross- talk.

Precise Spectral Imaging

The spectral detection unit uses a grating method that offers linear dispersion compared with prism dispersion. The unit provides 2 nm wavelength resolution to high-sensitivity photomultiplier tube detectors. Fluorescence separation can be achieved through unmixing, even when cross-talk is generated by multiple fluorescent dyes with similar peaks.

EGFP–EYFP Fluorescence Separation

2,600

2,400

2,200

2,000

	1,800						EYFP
Intensity	1,600
	1,400										EGFP
	1,200
	1,000
	800
	600
	400
	496	500	504	508	512	516	520	524	528	532	536	540	544	548	552
								Wavelength

EGFP (dendrite) — EYFP (synapse) XYλ

Wavelength detection range: 495 nm–561 nm in 2 nm steps

Excitation wavelength: 488 nm

Courtesy of: Dr. Shigeo Okabe Department of Anatomy and Cell Biology, Tokyo Medical and Dental University

Filter Based Detection

Enhanced Sensitivity

Three-channel scan unit with detection system featuring hard coated filter base. High-transmittance and high S/N ratio optical performance is achieved through integration of a pupil projection lens within the optics, the use of a high sensitivity photomultiplier and an analog processing circuit with minimal noise.

High-Performance Filters Deliver Outstanding Separation

Special coatings deliver exceptionally sharp transitions to a degree never achieved before, for acquisition of brighter fluorescence images.

DM488/543/633 Comparison

Transmittance (%)

100

80

60

40

20

0
480	500	520	540	560	580	600	620	640	660	680	700

Wavelength (nm)

Conventional mirror unit High-performance mirror unit

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