Technology / Hardware
SIM Scanner Unit for Simultaneous Laser Light Stimulation and Imaging.
SIM (Simultaneous) Scanner Unit
Combines the main scanner with a dedicated laser light stimulation scanner for investigating the trafficking of fluorescent- labeled molecules and marking of specific live cells.
Simultaneous Laser Light Stimulation and Imaging
Performs simultaneous laser light stimulation and imaging to acquire images of immediate cell responses to stimulation in photobleaching experiments.
Branching of laser in laser combiner.
AOTF
LD635
LD559AOTF
LD473
LD405
Lasers are used for both imaging and laser light stimulation.
Modifiable Stimulation Area During Imaging |
| Unique "Tornado" Scanning for Efficient Bleaching |
The stimulation area can be moved to a different position on the cell during imaging, providing a powerful tool for photoactivation and photoconversion experiments.
Conventional raster scanning does not always complete photobleaching quickly. Tornado scanning greatly improves bleaching efficiency by significantly reducing unnecessary scanning.
*Tornado scanning only available for SIM scanner.
Tornado scanning | ROI (region of interest) |
| scanning |
Superfluous scanning areas.
ROI (region of interest) scanning.
Tornado scanning.
Cell membrane stained with DIO, and subjected to both conventional ROI and tornado scanning.
Wide Choice of Bleaching Modes
Various scan modes can be used for both the observation area and stimulation area. Enables
Multi-Purpose Laser Combiner
All lasers can be used for both Imaging and laser light stimulation. LD405 / LD635 / AOTF / AOTF / LD473 / LD559
Laser Sharing with Main Scanner
Dual fiber laser combiner provides laser sharing between the SIM scanner and main scanner, eliminating the need to add a separate laser for stimulation.
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