Olympus FV1000 manual Scanning Units, Laser Systems, Illumination Units

Scanning Units

Two types of scanning units, filter-based and spectral detection, are provided. The design is all-in-one, integrating the scanning unit, tube lens and pupil projection lens. Use of the microscope fluorescence illuminator light path ensures that expandability of the microscope itself is not limited. Visible, UV and IR laser introduction ports are provided, as well as a feedback control system.

Scanning Unit for IX81 Inverted Microscope	Scanning Unit for BX61/BX61WI Upright Microscopes
Dedicated mirror unit cassette is required.	Fluorescence illuminator integrated with scanning unit.

Laser Systems

The multi-combiner enables combinations with all of the following diode lasers: 405 nm, 440 nm, 473 nm, 559 nm and 635 nm. The system can also be equipped with conventional Multi-line Ar laser and HeNe(G) laser.

Dual Type

The multi-combiner outputs laser light with two fibers. Light can be used both for observation and laser light stimulation.

Single Type

Single channel laser output. AOTF is standard equipment.

Illumination Units

Conventional illumination modules are designed for long-duration time-lapse experiments. Since light is introduced through fiber delivery systems, no heat is transferred to the microscope.

Fluorescence Illumination Unit

Stand with Mercury lamp house, motorized shutter, and fiber delivery system for conventional fluorescence observation. Light introduction via fiber optic port.

Transmitted Light Detection Unit

External transmitted light photomultiplier detector and 100 W Halogen conventional illumination, integrated for both laser scanning and conventional transmitted light Nomarski DIC observation. Motorized exchange between transmitted light illumination and laser detection. Simultaneous multi-channel confocal fluorescence image and transmitted DIC acquisition enabled.

Optional Upgrade Equipments for FV1000

4th Channel Detector Unit

Attaches to the optional port of either the filter or spectral type scanning unit and is used as a 4th confocal fluo- rescence detection channel. This is a filter-based fluorescence detection unit.

SIM Scanner

Second scanner dedicated for laser light stimulation, synchronized to the FV1000 main scanner for simultaneous laser light stimulation and confocal image acquisition. Independent fiber optic laser introduction port. Dichromatic mirror within motorized optical port of the scan unit required for introduction of laser into main scanner.

TIRFM Unit

Enables control of the necessary volume of excitation light using FV1000 soft- ware. This unit enables TIRF imaging using the laser light source used with Confocal.

Fiber Port for Fluorescence Output

Confocal fluorescence emission can be introduced via fiber delivery system into external device. Fiber port equipped with FC connector (fiber delivery system not included).

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