Contents

Contents

1.

Important Notes about this Manual

7

2.

Safety Notes

8

2.1.

General Safety Notes

8

2.2.

Electrical Safety

8

3.

Overview of the Instrument

10

4.

Unpacking the Microscope

14

5.

Assembling the Microscope

16

5.1

Stage

17

5.2

Condenser

18

5.3

Tube and Eyepieces

19

5.4

Objectives

19

5.5

Light Sources for the

 

 

Transmitted Light Axis

20

5.6

Light Sources for the

 

 

Incident Light Axis

21

5.7

Equipping the

 

 

Incident Light filter turret

26

5.8

Polarizer and Analyzer

27

5.9

DIC Prisms

28

5.10

Optional Accessories

29

5.11 Connection to the Power Supply

30

5.12 Connection to the

 

 

CTR5000 Electronics Box

30

6.

Startup

31

6.1

Functional Principle

31

6.2

Switching on the Microscope

34

6.3The Display

 

(Leica DM4000 B/DM4000 M)

35

6.4

The Function Keys

36

6.5

Köhler Illumination

37

6.6.

Checking Phase Contrast Rings

39

6.7

Adjusting the Light Sources

40

7.

Operation

46

7.1

Switching on the Microscope

46

7.2

Stages and Specimen Displacement ....

46

7.3

Focusing

47

7.4

Tubes

 

48

 

 

7.5

Eyepieces

49

7.6

Objectives

50

7.7

Magnification Changer

51

7.8

Light Sources

52

7.9Aperture Diaphragm and

Field Diaphragm

52

8.Imaging Procedure for

Leica DM4000 B/Leica DM5000 B

53

8.1 Transmitted Light

53

8.1.1

Bright Field

53

8.1.2

Phase Contrast

53

8.1.3

Dark Field

54

8.1.4

Polarization

55

8.1.5

Differential

 

 

Interference Contrast

56

5

Page 5
Image 5
Leica DM4000B, DM4000M manual Contents

DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.