10. Trouble Shooting

Problem

Cause/Remedy

 

 

Polarization

No polarization contrast is possible.

Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness (without specimen) (→ p. 55, 59).

Fluorescence

The image is completely dark (no fluorescence).

Open the shutter (→ p. 57).

Select the incident light axis (IL) (→ p. 36). Check the antigen-antibody combination.

The fluorescence is too weak.

Insert the Booster Lens (→ p. 29).

Center the lamp (→

p. 41ff).

Insert a new lamp (→

p. 20f).

Display

The display flashes.

Rotate an appropriate objective for the con-

 

trast method into the light path.

 

 

FAIL! appears.

Check insertion of objectives, cubes, etc.

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Image 63
Leica DM4000B, DM4000M manual Problem Cause/Remedy Polarization, Display

DM4000B, DM4000M specifications

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