8. Imaging Procedure for Leica DM4000 B/DM5000 B
8.2 Fluorescence
•Switch to the fluorescent light axis (FLUO) by pushing the TL/IL button.
•Insert a specimen and rotate a suitable objec- tive into place.
•The current fluorescence cube is indicated on the display.
•Closing the incident light shutter protects your specimen from fading.
Do so by pressing the SHUTTER variable key. (For key occupation please see “Identification Sheet”.)
The display indicates the symbol: ↓
•Selecting the fluorescence filter cube: Press the variable keys
Cube or Cube
•The fluorescence intensity can be increased using the Booster Lens on the right side of the stand (Fig. 69).
•For multifluorescence, use of a Excitation Manager is recommended. The Excitation Manager is inserted into the right side of the stand up to the last stop (Fig. 70).
•Using Booster Lens and Excitation Manager, the Excitation Manager can be inserted into the back receptacle.
Fig. 69 Inserting the Booster Lens | Fig. 70 Inserting the Excitation Manager |
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