Leica DM4000B, DM4000M manual Phlr

6. Startup

If your microscope is equipped for the use of phase contrast, the light rings that fit the objec- tives are built into the condenser.

The light rings are already leveled in the factory. However, the leveling should be rechecked.

Note:

Every objective is assigned its own light ring in the condenser disc. Therefore, a check must be performed for each objective. When swiveling in a suitable objective for phase contrast, the cor- responding light ring is set automatically.

•Press the BF (Bright Field) button (one of the variable function keys, behind the focus dials).

•In the place of an eyepiece, insert the focus- ing telescope (Fig. 42) into the observation tube.

•Swivel in the phase contrast objective with the least magnification.

•Focus the ring structure (43a) by slightly loos- ening the clamping ring (42.2) and moving the eyelens (42.1).

•Retighten the clamping ring.

•Press the PH (Phase Contrast) button. The ring diaphragm in the condenser is pivoted in.

•If the light ring and the phase ring are not shown as arranged in Fig. 43c, the light ring must be leveled.

Fig. 42 Focusing telescope		Fig. 43 Phase contrast centering procedure
1	Adjustable eyelens	PH=phase contrast ring, LR=light ring
2	Clamping ring for fixing the focus position	a Condenser in bright field (BF) position
		b Condenser in phase contrast (PH) position
		Light ring (LR) not centered
		c Light ring and phase ring centered

1	A	B	C
2

PHLR

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