6. Startup

The display shows the current microscope set- tings. The display depends on the microscope’s configuration. In the first column, corresponding pictograms indicate the type of information: con- trast method, magnification, light intensity, dia- phragms, light splitting for photo tubes.

Please see the abbreviation index for a list of ab- breviations and pictograms used → p. 67.

Light Intensity

The actual brightness setting is graphically de- picted by a beam. Additionally, the light intensity is indicated in 20 (coarse adjustment) or in 255 (fine adjustment) increments → p. 52.

Diaphragms

Contrast Method

In the first row, you find an indication of the ac- tive light axis (transmitted light or incident light) of the current contrast method and the current filter cube.

The shutter status is displayed for the transmitted light or incident light shutter:

Transmitted light shutter open

↑Transmitted light shutter closed

↑

Incident light shutter open

↓Incident light shutter closed

Magnification

+

The current objective magnification, sometimes followed by the re-magnification of the magnifi- cation changer, appears along with the total magnification:

Σ= Objective x Re-magnification x Eyepiece

6.4 The Function Keys

The values for the field diaphragm (FD) and the aperture diaphragm (AP) are indicated numeri- cally. The field diaphragm may be either round or rectangular. Accordingly, the FD designation is set in parentheses or in brackets: (FD) or [FD].

Note:

When using a digital camera, rectangular field diaphragms are recommended.

Beam splitting

If a motorized tube is used, the light splitting between ocular (Eye) and photo output (Docu) is indicated in %.

Note:

The display may flash after the initialization phase or even during microscopy session. This always occurs when the contrast method selected can not be performed with the actual microscopic settings. For example, an objective may be swiveled in that is not suited to the contrast method chosen.

Then check your settings.

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Leica DM4000B, DM4000M manual Light Intensity, Diaphragms Contrast Method, Magnification, Function Keys, Beam splitting
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DM4000B, DM4000M specifications

The Leica DM4000M and DM4000B are state-of-the-art microscopes designed for professional use in the fields of biology, materials science, and clinical applications. Renowned for their precision and innovative features, these instruments are perfect for researchers and clinicians needing high-resolution imaging capabilities.

One of the standout features of both models is the advanced motorized focusing system, which allows for swift adjustments and precise control. This feature is particularly useful in time-sensitive research environments, where accuracy and speed are paramount. The ergonomically designed focus mechanism promotes user comfort during prolonged observation sessions.

Both the DM4000M and DM4000B incorporate the revolutionary Leica Application Suite (LAS) software. This intuitive platform is designed to maximize the functionality of the microscope, enabling users to capture, analyze, and share images seamlessly. The software’s integrated tools are perfect for documenting findings and enhancing research productivity.

Another notable characteristic of the DM4000 series is the modular design, which allows for easy customization and upgrading. This aspect ensures that users can tailor their microscopes to meet specific research needs, whether it be for fluorescence microscopy, phase contrast, or even special imaging techniques like HSR or IR.

The high-performance optics provide exceptional image contrast and clarity, allowing users to observe minute details in samples. The combination of high numerical aperture objectives and advanced optical coatings enhances the resolution, making the DM4000 series ideal for examining intricate biological specimens as well as materials with complex textures.

The DM4000B model is particularly suited for routine clinical applications, featuring specific tools designed for rapid diagnosis and efficient workflows. Its user-friendly interface and dedicated clinical applications streamline laboratory processes, making it an essential device in any clinical setting.

Additionally, both models are equipped with LED illumination, which offers consistent light intensity and color temperature. This feature improves sample clarity while reducing heat generation, thereby protecting sensitive specimens during prolonged observation periods.

In conclusion, the Leica DM4000M and DM4000B microscopes represent a blend of advanced technology, intuitive design, and high adaptability. Their user-focused features and exceptional optical performance make them indispensable tools for researchers and clinical professionals aiming for excellence in microscopy.
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