9. Imaging Procedure for Leica DM4000 M
9.1.4 Interference Contrast
•Switch to the incident light axis (IL) by pushing the TL/IL button.
•Insert a specimen and rotate a suitable objec- tive into place.
•Select the DIC contrast method.
Do so by pressing the DIC variable key. Alternatively: Press the CHANGE RL variable key.
(For key occupation please see “Identification Sheet”.)
The display indicates ICR.
•The ICR filter cube (containing polarizer and analyzer) is automatically brought into the light path on the incident light axis. Insert the objec- tive prism slide into the prism slot (71.2).
Alternatively:
•Rotate the ICR polarizer (71.3) and the IC/P analyzer (72.1) on the stand manually into the light path.
•Insert the objective prism slide into the prism slot (71.2).
•For fine adjustment, rotate the knurled screw (71.1) on the objective prism slide.
9.2Transmitted Light 9.2.1 Bright Field
•Switch to the transmitted light axis by pushing the TL/IL button.
•Select the BF (bright field) contrast method. Do so by pressing the BF variable key. Alternatively: Press the CHANGE RL variable key.
(For key occupation please see “Identification Sheet”.)
The display indicates BF.
•Insert a transmitted light specimen.
•Rotate an appropriate objective into place.
•Use the focus dial to bring the image into fo- cus and set the brightness using the INT func- tion key.
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