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6. Startup
• Open the field diaphragm just enough for it to
disappear from the field of view (41d).
Caution:
Do not adjust the aperture diaphragm. The aper-
ture diaphragm is already set optimally for each
objective.
6.6. Checking Phase Contrast Rings
ABCD
Fig. 41 Köhler Illumination
aField diaphragm not focused, not centered
bField diaphragm focused, but not centered
cField diaphragm focused and centered
Diameter is too small, however
dField diameter (light) = Field diameter (view)
(Köhler Illumination)
Fig. 40 Condenser centering
1Centering bolts
11