Agilent Technologies G1978B setup guide Preparation

2Verification

To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes

To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes

N O T E	The G6110A instrument does not support the multimode source without the Selection
	valve. Use the option kit G2735H to enable the G6110A to work with the multimode source.

Preparation

1Transfer 1 mL of 5 ng/µL reserpine (Agilent G2423A) to a 50 mL volumetric flask. Use a clean graduated pipette.

2Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or acetic acid.

3Transfer 1 mL of the first dilution to a second 50 mL volumetric flask. Use a clean graduated pipette.

4Dilute to the 50 mL mark with 75:25 methanol/water with 0.1% formic or acetic acid.

This provides the final 2 pg/µL reserpine concentration required for performance verification.

5Transfer approximately 1 mL of the second dilution to a vial for use in the LC autosampler.

Table 1	6110 and 6120 Quad Performance Verification Summary Table
		G1978B	G1978B
		ES Positive SIM Mode	APCI Positive SIM Mode
Sample		Reserpine, 5 ng/µL	Reserpine, 5 ng/µL
Concentration after dilution		2 pg/µL	2 pg/µL
Injection volume		5 µL	5 µL
Total sample amount injected		10 pg	10 pg
Sample order number		G2423A	G2423A
Solvent		75:25	75:25
		methanol/water	methanol/water
		with 0.1% formic or acetic acid	with 0.1% formic or acetic acid

22 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide