Agilent Technologies G1978B setup guide Create and run a Multiple FIA Method Sequence

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2Verification

To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation

To verify sensitivity for the G1978B Multimode using Multiple FIA method in Mixed Mode operation

Preparation

1Do an autotune if necessary.

After the autotune has completed, you may need to wait up to 30 minutes before continuing to allow the calibrant solution to be pumped out of the Quad instrument. This minimizes any background signal from the calibrant.

You may need to further optimize the nebulizer pressure to achieve maximum instrument sensitivity.

2Load the method MMCheckSL_ES.M and:

a Edit the method to ensure that 65:35 methanol/water with .2% acetic acid is selected as the LC solvent.

b Save the method.

3Repeat for MMCheckSL_CI.M and MMCheckSL_MX.M.

4Place the vial into the LC autosampler.

•Position 21: vial with the ESI + APCI LC Demo Sample (p/n G1978-85000)

Create and run a Multiple FIA Method Sequence

1Select RunControl > Run Multiple FIA Methods.

2In the Run Multiple FIA Methods dialog box, click Group > Add Group.

3Type in a unique directory name for the data files to be stored.

4In the Run Multiple FIA Methods dialog box, click Methods > Add Method, select MMCheckSL_ES.M and click OK.

5Repeat to add MMCheckSL_CI.M.

6Repeat to add MMCheckSL_MX.M.

7Click Data File for the MMCheckSL_ES.M data file, then:

a Change the Subdirectory value to a unique subdirectory name in which to store the data files.

b Change Data File to Multi_ES.

c Change Operator to the user’s name or identification code.

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Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide

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Contents Set-Up Guide U T I O N WA R N I N G This Guide Page Content Contents Installation Installation Prepare to installInstall the HV control PCA and cables From left to right G1946-80058, G1960-60802 and G1960-60856Connecting the valve PCA to the multimode HV power supply Connecting the Vcap and Vchamber cables to the power supply Connecting the HV PCA to the chassis Changing Sources To remove the multimode sourceTo convert from multimode to ESI, Apci or Appi Installation on To convert from ESI, Apci or Appi to the multimode sourceStandard spray shield and capillary cap for ESI or Apci T E Multimode Spray Chamber No nebulizer on top of the multimode source LC/MS sample tubing connected to LC/MS inlet filter Verification Preparation 6110 and 6120 Quad Performance Verification Summary Table 6140 Quad Performance Verification Summary, SIM Mode 20 1 pk-pk 10 1 pk-pk Verification 6140 Quad Performance Verification Summary, Scan Mode 6130SCNESMM.M 6130SCNCIMM.M Verification Create and run a Multiple FIA Method Sequence Verification To do an autotune Autotune reportExample of multimode verification report Example of multimode verification report Methods To setup a method to use the multimode source Be impaired and the system may catch fireMethods Polarity options for Signal 1 and Signal To create a method for alternating ESI and Apci operation Method Spray Chamber set to MM-ES+APCIIonization mode for Signal 1 and Signal Index Index Page G1978-90070

G1978B specifications

Agilent Technologies G1978B is a high-performance mass spectrometer that is widely recognized for its innovations in analytical chemistry. This instrument stands out due to its combination of advanced technologies that enhance sensitivity, accuracy, and precision for complex analyses.

One of the key features of the G1978B is its compatibility with various ionization techniques, including Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI). This versatility allows users to analyze a diverse range of sample types, from small molecules to large biomolecules. Each ionization technique has its unique advantages, making the G1978B an excellent choice for laboratories focusing on pharmaceuticals, environmental analysis, and proteomics.

The G1978B is equipped with a sophisticated quadrupole mass filter, ensuring high-resolution mass measurements. This technology provides excellent mass accuracy and reproducibility, enabling users to distinguish between closely related compounds with minimal interference. The advanced data acquisition systems incorporated in the G1978B allow for rapid scanning capabilities, making it particularly effective for high-throughput screening applications.

In addition, the instrument features an intuitive user interface that simplifies method development and operational tasks. This includes streamlined software solutions that facilitate data analysis and interpretation, ensuring that even complex datasets can be managed efficiently. Furthermore, built-in diagnostic tools help in monitoring instrument performance, significantly reducing downtime and enhancing productivity.

The G1978B also incorporates advanced technologies for enhanced sensitivity. Its innovative source design minimizes background noise while maximizing signal intensity, making it particularly valuable for the detection of trace-level contaminants or low-abundance metabolites. Coupled with its exceptional dynamic range, the G1978B can quantify analytes over several orders of magnitude, which is crucial for applications in toxicology and clinical research.

With a robust design and ease of integration into existing laboratory workflows, the Agilent G1978B provides an ideal solution for researchers seeking a reliable mass spectrometry platform. Its combination of advanced ionization techniques, high-resolution capabilities, and user-friendly software makes it a versatile tool in modern analytical laboratories, paving the way for breakthroughs in research and development across a multitude of scientific disciplines.
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